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Literature summary for 3.5.1.2 extracted from

  • More, S.; Swamy, R.; Mohan, N.; Navyashree, M.; Janardhan, B.; Niyonzima, F.
    Purification and characterization of anti-cancer L-glutaminase of Bacillus cereus strain LC13 (2018), Proc. Natl. Acad. Sci. India: Sect. B Biol. Sci., 88, 695-705 .
No PubMed abstract available

Application

Application Comment Organism
synthesis optimal conditions for native enzyme production are pH 7.0,37°C and 0.3 % L-glutamine. Maltose (0.4 %, w/v) is best carbon source supplement Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 81% of initial activity Bacillus cereus
Cu2+ 5 mM, 86% of initial activity Bacillus cereus
dithiothreitol 5 mM, 82% of initial activity Bacillus cereus
Fe2+ 5 mM, 83% of initial activity Bacillus cereus
Fe3+ 5 mM, 84% of initial activity Bacillus cereus
Hg2+ 5 mM, 61% of initial activity Bacillus cereus
iodoacetamide 5 mM, 3% of initial activity Bacillus cereus
additional information phenylmethylsulfonyl fluoride, sodium azide, ethylenediaminetetraacetic acid and N-acetylimidazole have no effect on enzyme Bacillus cereus
N-bromosuccinimide 5 mM, 19% of initial activity Bacillus cereus
N-ethylmaleimide 5 mM, 8% of initial activity Bacillus cereus
TLCK 5 mM, 12% of initial activity Bacillus cereus
Zn2+ 5 mM, 79% of initial activity Bacillus cereus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Bacillus cereus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 5 mM, 113% of initial activity Bacillus cereus
Na+ 5 mM, 114% of initial activity Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus LC13
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58.6
-
pH 7.0, 37°C Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + H2O
-
Bacillus cereus L-glutamate + NH3
-
?
L-glutamine + H2O
-
Bacillus cereus LC13 L-glutamate + NH3
-
?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
Bacillus cereus