Protein Variants | Comment | Organism |
---|---|---|
M121C | about 2.5fold reduced activtiy | Helicobacter pylori |
M121C/T169M | mutant has a preserved efficiency vs L-asparagine but is completely unable to carry out L-glutamine hydrolysis. The mutant does not exert any cytotoxic effect on HL-60 cells | Helicobacter pylori |
Q63E | mutant displays similar catalytic efficiency versus asparagine and halved glutaminase efficiency with respect to the wild type enzyme, is able to exert a cytotoxic effect comparable to, or higher than, the one of the wild type enzyme | Helicobacter pylori |
T169M | about 4fold reduced catalytic activity | Helicobacter pylori |
T16D | replacement of catalytic threonine, depletes the enzyme of its catalytic activities with L-asparagine and L-glutamine | Helicobacter pylori |
T95D | replacement of catalytic threonine, depletes the enzyme of its catalytic activities with L-asparagine and L-glutamine | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-asparagine | mutant Q63E, pH 7.5, 37°C | Helicobacter pylori | |
0.23 | - |
L-asparagine | mutant M121C/T169M, cooperative kinetic toward L-Asn, Hill coefficient 1.7, pH 7.5, 37°C | Helicobacter pylori | |
0.25 | - |
L-asparagine | mutant M121C, cooperative kinetic toward L-Asn, Hill coefficient 1.5, pH 7.5, 37°C | Helicobacter pylori | |
0.25 | - |
L-asparagine | mutant T169M, cooperative kinetic toward L-Asn, Hill coefficient 1.8, pH 7.5, 37°C | Helicobacter pylori | |
0.29 | - |
L-asparagine | wild-type, pH 7.5, 37°C | Helicobacter pylori | |
46.4 | - |
L-glutamine | wild-type, cooperative kinetic toward L-Gln, Hill coefficient 2.0, pH 7.5, 37°C | Helicobacter pylori | |
76.5 | - |
L-glutamine | mutant Q63E, cooperative kinetic toward L-Gln, Hill coefficient 2.3, pH 7.5, 37°C | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | A0A2X5A091 | - |
- |
Helicobacter pylori ATCC 43504 | A0A2X5A091 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | reaction of EC 3.5.1.1 | Helicobacter pylori | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | reaction of EC 3.5.1.1 | Helicobacter pylori ATCC 43504 | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | - |
Helicobacter pylori | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Helicobacter pylori ATCC 43504 | L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AnsB | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
L-glutamine | wild-type, pH 7.5, 37°C | Helicobacter pylori | |
4.83 | - |
L-asparagine | mutant M121C, pH 7.5, 37°C | Helicobacter pylori | |
6.53 | - |
L-asparagine | mutant Q63E, pH 7.5, 37°C | Helicobacter pylori | |
7.4 | - |
L-asparagine | mutant T169M, pH 7.5, 37°C | Helicobacter pylori | |
13.95 | - |
L-asparagine | mutant M121C/T169M, pH 7.5, 37°C | Helicobacter pylori | |
14.5 | - |
L-glutamine | mutant Q63E, pH 7.5, 37°C | Helicobacter pylori | |
19.2 | - |
L-asparagine | wild-type, pH 7.5, 37°C | Helicobacter pylori |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
L-glutamine | mutant Q63E, cooperative kinetic toward L-Gln, Hill coefficient 2.3, pH 7.5, 37°C | Helicobacter pylori | |
0.47 | - |
L-glutamine | wild-type, cooperative kinetic toward L-Gln, Hill coefficient 2.0, pH 7.5, 37°C | Helicobacter pylori | |
29.6 | - |
L-asparagine | mutant T169M, cooperative kinetic toward L-Asn, Hill coefficient 1.8, pH 7.5, 37°C | Helicobacter pylori | |
30.2 | - |
L-asparagine | mutant M121C, cooperative kinetic toward L-Asn, Hill coefficient 1.5, pH 7.5, 37°C | Helicobacter pylori | |
60.7 | - |
L-asparagine | mutant M121C/T169M, cooperative kinetic toward L-Asn, Hill coefficient 1.7, pH 7.5, 37°C | Helicobacter pylori | |
65.3 | - |
L-asparagine | mutant Q63E, pH 7.5, 37°C | Helicobacter pylori | |
66.4 | - |
L-asparagine | wild-type, pH 7.5, 37°C | Helicobacter pylori |