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Literature summary for 3.5.1.2 extracted from

  • Bazaraa, W.; Alian, A.; El-Shimi, N.; Mohamed, R.
    Purification and characterization of extracellular glutaminase from Aspergillus oryzae NRRL 32567 (2016), Biocatal. Agricult. Biotechnol., 6, 76-81 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
NaCl 2.5%, 130% of initial activity, 10%, 109% of initial activity Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 0.1 mM, 55% of initial activity Aspergillus oryzae
Fe3+ 0.1 mM, 45% of initial activity Aspergillus oryzae
Mn2+ 0.1 mM, 75% of initial activity Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5
-
L-glutamine pH 7.0, 37°C Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus oryzae
-
-

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Aspergillus oryzae NRRL 32567
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51
-
pH 7.0, 37°C Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glutamine + H2O
-
Aspergillus oryzae D-glutamate + NH3
-
?
D-glutamine + H2O
-
Aspergillus oryzae NRRL 32567 D-glutamate + NH3
-
?
L-asparagine + H2O reaction of EC 3.5.1.1 Aspergillus oryzae L-aspartate + NH3
-
?
L-asparagine + H2O reaction of EC 3.5.1.1 Aspergillus oryzae NRRL 32567 L-aspartate + NH3
-
?
L-glutamine + H2O
-
Aspergillus oryzae L-glutamate + NH3
-
?
L-glutamine + H2O
-
Aspergillus oryzae NRRL 32567 L-glutamate + NH3
-
?

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25
-
40% of maximum activity Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
stable for at least 2 h Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Aspergillus oryzae