Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 138.22% activity at 100 mM | Bacillus cereus | |
cysteine | 128.15% activity at 100 mM | Bacillus cereus | |
dithiothreitol | 125.13% activity at 100 mM | Bacillus cereus | |
glutathione | 129.32% activity at 100 mM | Bacillus cereus | |
L-ascorbic acid | 128.23% activity at 100 mM | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 72.59% residual activity at 100 mM | Bacillus cereus | |
Cd2+ | complete inhibition at 50 mM | Bacillus cereus | |
Co2+ | 70.53% residual activity at 100 mM | Bacillus cereus | |
Cu2+ | complete inhibition at 50 mM | Bacillus cereus | |
Hg2+ | complete inhibition at 50 mM | Bacillus cereus | |
iodoacetamide | complete inhibition at 50 mM | Bacillus cereus | |
Mg2+ | 74.29% residual activity at 100 mM | Bacillus cereus | |
Mn2+ | 76.23% residual activity at 100 mM | Bacillus cereus | |
additional information | not inhibited by EDTA | Bacillus cereus | |
p-chloromercuribenzoic acid | complete inhibition at 10 mM | Bacillus cereus | |
Pb2+ | 69.27% residual activity at 100 mM | Bacillus cereus | |
Zn2+ | 82.12% residual activity at 100 mM | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.25 | - |
L-glutamine | at pH 7.5 and 35°C | Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 139.43% activity at 100 mM | Bacillus cereus | |
Na+ | 120.35% activity at 100 mM | Bacillus cereus | |
PO43- | 143.13% activity at 100 mM | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Bacillus cereus |
140000 | - |
gel filtration | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Bacillus cereus | - |
L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | Bacillus cereus MTCC 1305 | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus MTCC 1305 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography | Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.502 | - |
crude extract, at pH 7.5 and 35°C | Bacillus cereus |
7.44 | - |
after 14.8fold purification, at pH 7.5 and 35°C | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glutamine + H2O | 48.5% activity compared to L-glutamine | Bacillus cereus | D-glutamate + NH3 | - |
? | |
D-glutamine + H2O | 48.5% activity compared to L-glutamine | Bacillus cereus MTCC 1305 | D-glutamate + NH3 | - |
? | |
L-glutamic acid + H2O | 13.5% activity compared to L-glutamine | Bacillus cereus | ? | - |
? | |
L-glutamic acid + H2O | 13.5% activity compared to L-glutamine | Bacillus cereus MTCC 1305 | ? | - |
? | |
L-glutamine + H2O | - |
Bacillus cereus | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | 100% activity | Bacillus cereus | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Bacillus cereus MTCC 1305 | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | 100% activity | Bacillus cereus MTCC 1305 | L-glutamate + NH3 | - |
? | |
additional information | the enzyme does not show any relative activity against L-asparagine, D-asparagine, and L-gamma-glutamyl-4-nitroanilide | Bacillus cereus | ? | - |
? | |
additional information | the enzyme does not show any relative activity against L-asparagine, D-asparagine, and L-gamma-glutamyl-4-nitroanilide | Bacillus cereus MTCC 1305 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 35000, SDS-PAGE | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
L-glutaminase | - |
Bacillus cereus |
L-glutamine amidohydrolase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | the enzyme shows relative activity above 70% for temperature range from 30 to 45°C and inactivation of enzyme at higher temperature above 50°C | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | the enzyme retains relative stability up to 95% from 20 to 35°C and 50% from 40 to 50°C for 30 min. The stability of enzyme decreases sharply at higher temperature | Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.222 | - |
L-glutamine | at pH 7.5 and 35°C | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | more than 70% relative activity in pH range from 7.0 to 8.0. Loss of activity is elevated at higher pH (above 8.0), and the enzyme retains 27% of optimum activity when assayed at pH 8.5 | Bacillus cereus |