BRENDA - Enzyme Database
show all sequences of 3.5.1.2

A temperature and salt-tolerant L-glutaminase from Gangotri region of uttarakhand himalaya: enzyme purification and characterization

Kumar, L.; Singh, B.; Adhikari, D.K.; Mukherjee, J.; Ghosh, D.; Appl. Biochem. Biotechnol. 166, 1723-1735 (2012)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
NaCl
enhances activity at 1-20% w/v, but reduces activity by 30% at 25% w/v, at 25% NaCl concentration, the enzyme retains 60% of its activity after 4 h
Bacillus sp. (in: Bacteria)
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
L-glutamine
pH 11.0, 70°C
Bacillus sp. (in: Bacteria)
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
NaCl
enhances activity at 1-20% w/v, but reduces activity by 30% at 25% w/v, optimal at 1%
Bacillus sp. (in: Bacteria)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
x * 66000, SDS-PAGE
Bacillus sp. (in: Bacteria)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-glutamine + H2O
Bacillus sp. (in: Bacteria)
-
L-glutamate + NH3
-
-
?
L-glutamine + H2O
Bacillus sp. (in: Bacteria) LKG-01
-
L-glutamate + NH3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus sp. (in: Bacteria)
-
MTCC 10401, isolated from Gangotri region of Uttarakhand Himalaya
-
Bacillus sp. (in: Bacteria) LKG-01
-
MTCC 10401, isolated from Gangotri region of Uttarakhand Himalaya
-
Purification (Commentary)
Commentary
Organism
native enzyme, 49fold from cell-free extract by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, to homogeneity
Bacillus sp. (in: Bacteria)
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
584.2
-
purified enzyme, pH 11.0, 70°C
Bacillus sp. (in: Bacteria)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glutamine + H2O
low activity
718613
Bacillus sp. (in: Bacteria)
D-glutamate + NH3
-
-
-
?
D-glutamine + H2O
low activity
718613
Bacillus sp. (in: Bacteria) LKG-01
D-glutamate + NH3
-
-
-
?
L-glutamine + H2O
-
718613
Bacillus sp. (in: Bacteria)
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
preferred substrate
718613
Bacillus sp. (in: Bacteria)
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
-
718613
Bacillus sp. (in: Bacteria) LKG-01
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
preferred substrate
718613
Bacillus sp. (in: Bacteria) LKG-01
L-glutamate + NH3
-
-
-
?
L-glutaminyl-L-asparagine + H2O
-
718613
Bacillus sp. (in: Bacteria)
?
-
-
-
?
L-glutaminyl-L-asparagine + H2O
-
718613
Bacillus sp. (in: Bacteria) LKG-01
?
-
-
-
?
additional information
no activity with D-asparagine
718613
Bacillus sp. (in: Bacteria)
?
-
-
-
-
additional information
no activity with D-asparagine
718613
Bacillus sp. (in: Bacteria) LKG-01
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 66000, SDS-PAGE
Bacillus sp. (in: Bacteria)
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Bacillus sp. (in: Bacteria)
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
35
85
activity range
Bacillus sp. (in: Bacteria)
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
purified enzyme, 3 h, 100% stable
Bacillus sp. (in: Bacteria)
70
-
purified enzyme, 3 h, 50% activity remaining
Bacillus sp. (in: Bacteria)
80
-
purified enzyme, 3 h, 20% activity remaining
Bacillus sp. (in: Bacteria)
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
-
Bacillus sp. (in: Bacteria)
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
12
activity range
Bacillus sp. (in: Bacteria)
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
11
purified enzyme, stable
Bacillus sp. (in: Bacteria)
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NaCl
enhances activity at 1-20% w/v, but reduces activity by 30% at 25% w/v, at 25% NaCl concentration, the enzyme retains 60% of its activity after 4 h
Bacillus sp. (in: Bacteria)
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.24
-
L-glutamine
pH 11.0, 70°C
Bacillus sp. (in: Bacteria)
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
NaCl
enhances activity at 1-20% w/v, but reduces activity by 30% at 25% w/v, optimal at 1%
Bacillus sp. (in: Bacteria)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
x * 66000, SDS-PAGE
Bacillus sp. (in: Bacteria)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-glutamine + H2O
Bacillus sp. (in: Bacteria)
-
L-glutamate + NH3
-
-
?
L-glutamine + H2O
Bacillus sp. (in: Bacteria) LKG-01
-
L-glutamate + NH3
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme, 49fold from cell-free extract by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, to homogeneity
Bacillus sp. (in: Bacteria)
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
584.2
-
purified enzyme, pH 11.0, 70°C
Bacillus sp. (in: Bacteria)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glutamine + H2O
low activity
718613
Bacillus sp. (in: Bacteria)
D-glutamate + NH3
-
-
-
?
D-glutamine + H2O
low activity
718613
Bacillus sp. (in: Bacteria) LKG-01
D-glutamate + NH3
-
-
-
?
L-glutamine + H2O
-
718613
Bacillus sp. (in: Bacteria)
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
preferred substrate
718613
Bacillus sp. (in: Bacteria)
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
-
718613
Bacillus sp. (in: Bacteria) LKG-01
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
preferred substrate
718613
Bacillus sp. (in: Bacteria) LKG-01
L-glutamate + NH3
-
-
-
?
L-glutaminyl-L-asparagine + H2O
-
718613
Bacillus sp. (in: Bacteria)
?
-
-
-
?
L-glutaminyl-L-asparagine + H2O
-
718613
Bacillus sp. (in: Bacteria) LKG-01
?
-
-
-
?
additional information
no activity with D-asparagine
718613
Bacillus sp. (in: Bacteria)
?
-
-
-
-
additional information
no activity with D-asparagine
718613
Bacillus sp. (in: Bacteria) LKG-01
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 66000, SDS-PAGE
Bacillus sp. (in: Bacteria)
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
-
Bacillus sp. (in: Bacteria)
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
35
85
activity range
Bacillus sp. (in: Bacteria)
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
purified enzyme, 3 h, 100% stable
Bacillus sp. (in: Bacteria)
70
-
purified enzyme, 3 h, 50% activity remaining
Bacillus sp. (in: Bacteria)
80
-
purified enzyme, 3 h, 20% activity remaining
Bacillus sp. (in: Bacteria)
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
-
Bacillus sp. (in: Bacteria)
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
12
activity range
Bacillus sp. (in: Bacteria)
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
11
purified enzyme, stable
Bacillus sp. (in: Bacteria)
Other publictions for EC 3.5.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733460
Pandian
-
Optimization and purification ...
Alcaligenes faecalis, Alcaligenes faecalis KLU102
Biologia
69
1644-1651
2015
-
-
-
-
-
-
-
-
-
-
1
2
-
2
-
-
1
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733233
Karahan
Immobilization of glutaminase ...
Trichoderma reesei
Artif. Cells Nanomed. Biotechnol.
42
262-267
2014
-
-
-
-
-
1
-
2
-
-
-
1
-
2
-
-
-
-
-
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
-
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
734818
Lee
Discovery of selective inhibit ...
Homo sapiens
Oncotarget
5
6087-6101
2014
-
-
1
-
8
-
29
-
-
-
-
1
-
2
-
-
1
-
-
7
-
-
1
-
-
-
-
-
-
-
-
-
-
-
57
-
-
1
-
-
8
-
57
29
-
-
-
-
-
1
-
-
-
1
-
7
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
735259
Thangavelu
Structural basis for the activ ...
Homo sapiens
Sci. Rep.
4
3827
2014
-
-
1
1
4
-
4
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
4
-
-
4
-
-
-
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733133
Singh
Biochemical characterization a ...
Bacillus cereus, Bacillus cereus MTCC 1305
Appl. Biochem. Biotechnol.
171
522-531
2013
5
-
-
-
-
-
12
1
-
3
2
2
-
4
-
-
1
-
-
-
2
-
10
1
1
1
1
1
1
1
-
-
-
-
-
5
-
-
-
-
-
-
-
12
-
1
-
3
2
2
-
-
-
1
-
-
2
-
10
1
1
1
1
1
1
1
-
-
-
-
-
-
-
-
733187
Ito
Purification and characterizat ...
Aspergillus sojae
Appl. Microbiol. Biotechnol.
97
8581-8590
2013
-
-
1
-
-
-
1
-
-
-
3
2
-
4
-
1
-
-
-
-
8
-
16
1
1
-
-
3
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
3
2
-
-
1
-
-
-
8
-
16
1
1
-
-
3
1
-
1
-
-
-
-
-
-
-
733239
Pu
-
Cloning, expression of glutami ...
Pseudomonas nitroreducens, Pseudomonas nitroreducens DSM 14399
Biocatal. Biotransform.
31
1-7
2013
-
-
1
-
-
-
-
-
-
-
1
2
-
2
-
-
1
-
-
-
-
-
6
1
1
1
-
-
1
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
6
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
733262
Thomas
Kinetic characterization of eb ...
Mus musculus
Biochem. Biophys. Res. Commun.
438
243-248
2013
-
-
-
-
-
-
14
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
4
-
11
-
-
-
-
-
-
-
11
14
4
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
733562
Ye
-
Production of a novel salt-tol ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens Y-9
Biotechnology
12
25-35
2013
-
1
-
-
-
1
7
-
-
2
1
2
-
2
-
-
1
-
-
-
2
-
11
1
1
-
1
-
1
1
1
-
-
-
-
-
1
-
-
-
-
1
-
7
-
-
-
2
1
2
-
-
-
1
-
-
2
-
11
1
1
-
1
-
1
1
1
-
-
-
-
-
-
-
718613
Kumar
A temperature and salt-toleran ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) LKG-01
Appl. Biochem. Biotechnol.
166
1723-1735
2012
-
-
-
-
-
-
1
1
-
1
1
2
-
2
-
-
1
-
-
-
1
-
10
1
1
1
3
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
1
2
-
-
-
1
-
-
1
-
10
1
1
1
3
-
1
1
1
-
-
-
-
-
-
-
718925
List
Constitutively active glutamin ...
Thermotoga maritima
Biochemistry
51
2812-2818
2012
1
-
1
-
5
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
5
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
1
-
-
1
-
-
720930
Cassago
Mitochondrial localization and ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
109
1092-1097
2012
1
-
-
1
1
-
-
1
3
1
-
-
-
1
-
-
-
-
-
8
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
1
3
1
-
-
-
-
-
-
-
8
-
-
-
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
720942
Thangavelu
Structural basis for the allos ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
109
7705-7710
2012
1
-
1
-
1
-
1
-
-
-
-
1
-
2
-
1
-
-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
-
3
3
-
-
-
720975
Sinsuwan
Efficient expression and purif ...
Bacillus licheniformis, Bacillus licheniformis DSM 13
Protein Expr. Purif.
83
52-58
2012
-
1
1
-
-
-
-
1
-
-
3
2
-
4
-
-
1
-
-
-
1
1
2
1
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
3
2
-
-
-
1
-
-
1
1
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
734440
Shukla
Design, synthesis, and pharmac ...
Homo sapiens
J. Med. Chem.
55
10551-10563
2012
-
-
-
-
-
-
30
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
27
-
-
-
-
-
-
-
27
30
-
-
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-
-
-
-
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-
-
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-
685413
Montero
Glutaminase activity is confin ...
Rattus norvegicus
Biochimie
89
1366-1371
2007
-
-
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2
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1
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1
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-
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687117
Campos-Sandoval
Expression of functional human ...
Homo sapiens
Int. J. Biochem. Cell Biol.
39
765-773
2007
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-
1
-
-
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-
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688502
Erdmann
Glutamate production by HIV-1 ...
Homo sapiens
J. Neurochem.
102
539-549
2007
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-
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1
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5
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3
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1
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1
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3
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1
-
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-
-
689587
Tambasco-Studart
Functional analysis of PDX2 fr ...
Arabidopsis thaliana
Plant Physiol.
144
915-925
2007
-
-
1
-
-
-
-
-
-
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1
-
9
-
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1
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1
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1
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1
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1
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1
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-
667444
Yoshimune
Crystal structure of a major f ...
Micrococcus luteus, Micrococcus luteus K-3
Biochem. Biophys. Res. Commun.
346
1118-1124
2006
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-
1
1
2
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1
4
2
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7
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1
1
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2
2
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1
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2
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1
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2
2
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668902
Sivakumar
Optimum conditions for L-gluta ...
Streptomyces rimosus, Streptomyces rimosus LG-10
Indian J. Exp. Biol.
44
256-258
2006
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1
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1
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2
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5
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2
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669592
Buschdorf
Brain-specific BNIP-2-homology ...
Homo sapiens
J. Cell Sci.
119
3337-3350
2006
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1
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1
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1
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2
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5
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2
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1
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2
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3
-
-
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1
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-
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-
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669950
Masson
Mice lacking brain/kidney phos ...
Mus musculus
J. Neurosci.
26
4660-4671
2006
-
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1
-
1
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1
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1
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3
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5
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2
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1
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1
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1
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1
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1
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1
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5
-
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2
-
1
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-
1
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-
-
-
-
-
-
-
-
670391
Marquez
Glutaminase: a multifaceted pr ...
Homo sapiens, no activity in Gallus gallus, Rattus norvegicus
Neurochem. Int.
48
465-471
2006
-
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1
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4
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1
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9
1
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2
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4
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19
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5
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1
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4
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1
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9
1
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2
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19
-
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5
-
-
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-
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-
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687847
Yano
Analysis of essential amino ac ...
Micrococcus luteus, Micrococcus luteus K-3
J. Biosci. Bioeng.
102
362-364
2006
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1
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5
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1
4
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9
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4
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4
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2
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-
4
-
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-
-
690194
Romero-Gomez
Phosphate-activated glutaminas ...
Rattus norvegicus
World J. Gastroenterol.
12
2406-2411
2006
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-
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-
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6
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15
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15
-
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-
-
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-
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-
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-
-
-
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667496
Perez-Gomez
Co-expression of glutaminase K ...
Homo sapiens
Biochem. J.
386
535-542
2005
-
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1
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1
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1
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1
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6
-
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1
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1
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1
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1
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1
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1
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6
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
668317
Watford
-
Glutamine metabolism in uricot ...
Columba livia, Gallus gallus, Sceloporus undulatus
Comp. Biochem. Physiol. B
140B
607-614
2005
-
-
-
-
-
-
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3
-
3
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6
3
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3
-
3
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3
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-
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3
-
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6
3
-
3
-
3
-
-
-
3
-
-
-
-
-
-
-
-
-
669542
Masuo
Micrococcus luteus K-3-type gl ...
Aspergillus oryzae
J. Biosci. Bioeng.
100
576-578
2005
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1
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1
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2
1
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1
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4
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-
-
-
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-
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2
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1
1
1
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1
1
1
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1
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1
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2
1
-
1
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-
-
-
-
2
-
1
1
1
-
1
1
1
-
-
-
-
-
-
-
669739
Wakayama
Characterization of salt-toler ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia NYW-81
J. Ind. Microbiol. Biotechnol.
32
383-390
2005
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1
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6
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3
2
2
-
8
-
-
1
-
-
1
1
-
10
1
1
-
2
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
-
3
2
2
-
-
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1
-
1
1
-
10
1
1
-
2
-
1
-
1
-
-
-
-
-
-
-
670129
Romero-Gomez
Role of phosphate-activated gl ...
Homo sapiens
Metab. Brain Dis.
20
319-325
2005
2
-
-
-
-
-
1
-
-
1
-
1
-
2
-
-
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-
-
11
-
-
2
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2
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-
-
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1
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1
-
1
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-
11
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654884
Huerta-Saquero
Regulation of transcription an ...
Rhizobium etli
Biochim. Biophys. Acta
1673
201-207
2004
2
-
1
-
-
-
4
-
-
1
-
1
-
2
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1
1
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1
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2
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2
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2
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1
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4
2
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1
-
1
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1
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656204
Castell
Granule localization of glutam ...
Homo sapiens
J. Biol. Chem.
279
13305-13310
2004
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1
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-
-
-
-
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1
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1
1
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2
-
1
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1
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2
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1
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1
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1
1
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1
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656222
Bauer
Three-dimensional structure of ...
Bacillus subtilis
J. Biol. Chem.
279
2704-2711
2004
-
-
1
1
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-
-
-
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1
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2
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1
1
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-
1
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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1
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-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667477
Baglietto-Vargas
Segregation of two glutaminase ...
Rattus norvegicus
Biochem. J.
381
483-487
2004
-
-
-
-
-
-
-
-
-
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2
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1
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1
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-
6
-
-
2
-
-
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-
-
-
-
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-
-
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-
-
-
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-
-
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2
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1
-
6
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668534
Yoshimune
Digestion by serine proteases ...
Micrococcus luteus, Micrococcus luteus K-3
Extremophiles
8
441-446
2004
-
-
1
-
-
-
1
4
-
1
3
2
-
10
-
-
1
-
-
-
2
-
2
1
1
-
-
5
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
4
-
1
3
2
-
-
-
1
-
-
2
-
2
1
1
-
-
5
1
-
-
-
-
-
-
-
-
-
669694
Romero-Gomez
Intestinal glutaminase activit ...
Homo sapiens
J. Hepatol.
41
49-54
2004
-
-
-
-
-
-
-
-
1
1
-
1
-
2
-
-
-
-
-
4
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
4
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670768
Masuo
Molecular cloning, overexpress ...
Aspergillus oryzae, Aspergillus oryzae RIB 40
Protein Expr. Purif.
38
272-278
2004
-
-
1
-
-
-
-
-
-
-
1
2
-
7
-
-
1
-
-
-
3
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
3
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
654091
Chantawannakul
Crystallization and preliminar ...
Micrococcus luteus, Micrococcus luteus K-3
Acta Crystallogr. Sect. D
59
566-568
2003
-
1
1
1
-
-
-
-
-
1
2
2
-
9
-
1
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
1
2
2
-
-
1
1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655373
Weingand-Ziade
-
Functional characterization of ...
Lactobacillus rhamnosus
Enzyme Microb. Technol.
32
862-867
2003
-
1
-
-
-
-
1
2
1
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
1
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657262
Kenny
Bacterial expression, purifica ...
Rattus norvegicus
Protein Expr. Purif.
31
140-148
2003
-
-
1
-
-
-
-
6
2
1
5
1
-
4
-
1
1
-
-
1
2
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
2
1
5
1
-
-
1
1
-
1
2
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655601
Klein
Isolation of a novel, phosphat ...
Sporosarcina pasteurii
FEMS Microbiol. Lett.
206
63-67
2002
-
-
-
-
-
-
1
7
1
4
2
1
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1
1
-
-
1
1
-
8
1
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
7
1
4
2
1
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1
1
-
1
1
-
8
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
654234
Sidel'nikova
-
Characterization of glutaminas ...
Triticale sp.
Appl. Biochem. Microbiol.
37
569-573
2001
-
-
-
-
-
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3
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2
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1
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1
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1
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1
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2
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-
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1
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-
-
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-
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-
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3
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2
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1
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1
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1
-
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2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
655542
Olalla
The C-terminus of human glutam ...
Homo sapiens
FEBS Lett.
488
116-122
2001
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1
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1
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1
1
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1
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1
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1
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-
-
-
-
-
-
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-
-
-
-
-
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1
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1
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1
1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
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-
-
-
209032
Nandakumar
Overexpression of salt-toleran ...
Micrococcus luteus
Protein Expr. Purif.
15
155-161
1999
-
-
1
1
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1
6
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4
1
1
-
7
-
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1
-
-
-
1
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
1
-
1
-
1
-
6
-
-
-
4
1
1
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209035
Maity
Isolation and purification of ...
Mus musculus
J. Exp. Clin. Cancer Res.
18
475-480
1999
1
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209027
Campos
Involvement of essential cyste ...
Mus musculus
Biochim. Biophys. Acta
1429
275-283
1998
1
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1
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209030
Newcomb
Characterization of mitochondr ...
Rattus norvegicus
Brain Res.
813
103-111
1998
1
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209034
Shenoy
Characterization of glutaminas ...
Rattus norvegicus
J. Nutr.
126
1121S-1130S
1996
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209036
Segura
Tumor glutaminase purification ...
Mus musculus
Protein Expr. Purif.
7
343-351
1995
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2
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666994
Lebedeva
-
Molecular and catalytic proper ...
Pseudomonas chlororaphis subsp. aurantiaca
Vestn. Akad. Med. Nauk SSSR
2
57-61
1995
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3
1
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1
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1
1
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209026
Moriguchi
-
Isolation and characterization ...
Micrococcus luteus, Micrococcus luteus K-3
J. Ferment. Bioeng.
77
621-625
1994
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2
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1
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8
1
1
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209031
Swierczynski
Phosphate-dependent glutaminas ...
Homo sapiens, Rattus norvegicus
Biochim. Biophys. Acta
1157
55-62
1993
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7
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7
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209028
Nelson
Glutamine catabolism by heart ...
Rattus norvegicus
Biochem. J.
282
559-564
1992
6
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4
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209033
Michalik
Glutamate production in islets ...
Rattus norvegicus
Metabolism
41
1319-1326
1992
2
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1
5
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209029
Shapiro
Isolation, characterization, a ...
Rattus norvegicus
J. Biol. Chem.
266
18792-18796
1991
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1
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3
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1
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209009
Heini
Purification and characterizat ...
Rattus norvegicus
Eur. J. Biochem.
162
541-546
1987
1
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1
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1
1
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-
209010
Kvamme
Glutaminase from mammalian tis ...
Rattus norvegicus, Sus scrofa
Methods Enzymol.
113
241-256
1985
2
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2
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2
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1
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4
2
1
2
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1
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4
2
1
2
2
-
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-
-
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-
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-
209011
Holcenberg
Glutaminase from Acinetobacter ...
Acinetobacter glutaminasificans
Methods Enzymol.
113
257-263
1985
-
-
-
1
-
1
4
2
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1
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2
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5
1
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1
5
1
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1
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-
209012
Haser
Comparison of the phosphate-de ...
Rattus norvegicus
Biochem. J.
229
399-408
1985
1
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1
2
1
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4
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4
1
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4
1
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1
1
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-
209013
Patel
Partial purification and prope ...
Rattus norvegicus
Biochem. J.
220
583-590
1984
2
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2
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2
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2
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1
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2
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1
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2
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1
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2
1
-
1
1
-
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-
1
-
-
-
-
-
-
-
-
-
209014
Snodgrass
Allosteric properties of phosp ...
Homo sapiens
Biochim. Biophys. Acta
798
21-27
1984
2
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1
1
2
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1
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2
-
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2
-
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-
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-
-
-
-
-
-
-
-
-
209015
Ardawi
Intracellular localization and ...
Rattus norvegicus
Biochem. J.
217
289-296
1984
11
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8
1
2
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15
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1
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11
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15
-
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1
-
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-
1
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-
-
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-
-
-
-
-
209016
Morehouse
Properties of rat renal phosph ...
Rattus norvegicus
Biochem. J.
193
709-716
1981
2
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1
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2
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2
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1
1
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1
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2
-
-
1
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
209017
McGivan
Localization and some properti ...
Rattus norvegicus
Biochem. J.
192
537-542
1980
3
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2
1
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1
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1
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1
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3
-
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2
1
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1
-
-
1
-
-
-
-
-
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-
-
-
-
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-
-
-
209018
Kvamme
Evidence for two species of ma ...
Rattus norvegicus, Sus scrofa
FEBS Lett.
107
33-36
1979
-
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-
-
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5
-
2
1
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2
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4
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2
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5
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1
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4
-
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2
-
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-
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-
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209019
Kovacevic
Molecular form and kinetic pro ...
Rattus norvegicus
Biochim. Biophys. Acta
567
216-224
1979
2
-
-
-
-
-
2
-
2
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2
-
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2
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1
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1
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2
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2
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2
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2
-
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1
-
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-
1
-
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-
-
-
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-
-
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-
209020
Oshima
-
Further characterization of gl ...
Pseudomonas aeruginosa
Agric. Biol. Chem.
40
2251-2256
1976
-
-
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5
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2
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1
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30
1
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5
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2
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30
1
-
-
-
-
-
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-
-
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-
209021
Prusiner
Regulation of glutaminase B in ...
Escherichia coli
J. Biol. Chem.
251
3447-3456
1976
-
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-
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2
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1
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2
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1
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1
1
2
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1
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2
1
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-
-
-
-
2
-
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-
1
-
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1
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1
1
2
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
209022
Abe
Reinvestigation of the physico ...
Pseudomonas sp.
Biochim. Biophys. Acta
358
113-116
1974
-
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1
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-
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3
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2
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-
1
1
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-
1
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-
1
-
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-
1
-
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-
-
-
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-
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3
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-
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-
-
-
-
1
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
209023
Katsumata
Purification and physicochemic ...
Pseudomonas sp.
Biochim. Biophys. Acta
289
405-409
1972
-
-
-
1
-
-
-
-
-
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1
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2
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1
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1
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1
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-
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-
1
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-
1
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1
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-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663903
Soda
Purification and properties of ...
Pseudomonas aeruginosa
Biochem. Biophys. Res. Commun.
46
1278-1284
1972
-
-
-
1
-
-
3
2
-
-
2
2
-
3
-
-
1
-
-
-
2
2
13
-
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-
-
1
1
-
-
-
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-
1
-
-
-
4
-
2
-
-
2
2
-
-
-
2
-
-
2
2
13
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
209024
Hartman
-
Glutaminases and gamma-glutamy ...
Escherichia coli
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
79-100
1971
3
-
-
-
-
-
5
9
-
-
-
-
-
1
-
-
1
1
-
-
-
-
18
-
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-
-
11
-
-
-
-
-
-
-
3
-
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-
-
-
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5
-
9
-
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1
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-
-
-
18
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
209025
Roberts
-
Glutaminase ...
Canis lupus familiaris, Clostridium welchii, Sus scrofa
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. )
4
285-300
1960
-
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-
-
-
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26
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3
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2
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2
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9
-
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2
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26
-
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2
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2
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9
-
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2
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