Cloned (Comment) | Organism |
---|---|
mouse nitrilase 2 is expressed as a His-tagged fusion protein in Escherichia coli and in HEK-293T cells | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
2-oxo-succinamate | pH 7.2, 30°C | Mus musculus | |
0.012 | - |
5-methoxy-2,5-dioxopentanoic acid | pH 7.2, 30°C | Mus musculus | |
0.017 | - |
2-oxo-succinamate | pH 8.5, 30°C | Mus musculus | |
0.14 | - |
succinamate | pH 8.5, 30°C | Mus musculus | |
0.195 | - |
2-oxo-glutaramate | pH 8.5, 30°C | Mus musculus | |
0.23 | - |
succinamate | pH 7.2, 30°C | Mus musculus | |
1.27 | - |
Glutaramate | pH 8.5, 30°C | Mus musculus | |
1.48 | - |
Glutaramate | pH 7.2, 30°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-glutaramate + H2O | - |
Mus musculus | ? | - |
? | |
2-oxo-succinamate + H2O | - |
Mus musculus | ? | - |
? | |
5-methoxy-2,5-dioxopentanoic acid + H2O | - |
Mus musculus | methanol + 2-oxoglutaric acid | - |
? | |
glutaramate + H2O | - |
Mus musculus | ? | - |
? | |
succinamate + H2O | - |
Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Nit 2 | - |
Mus musculus |
nitrilase 2 | - |
Mus musculus |
omega-amidase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.87 | - |
2-oxo-succinamate | pH 8.5, 30°C | Mus musculus | |
1.15 | - |
2-oxo-succinamate | pH 7.2, 30°C | Mus musculus | |
2 | - |
succinamate | pH 7.2, 30°C | Mus musculus | |
2.8 | - |
succinamate | pH 8.5, 30°C | Mus musculus | |
4.1 | - |
Glutaramate | pH 7.2, 30°C | Mus musculus | |
8.8 | - |
Glutaramate | pH 8.5, 30°C | Mus musculus | |
17.5 | - |
2-oxo-glutaramate | pH 8.5, 30°C | Mus musculus | |
134.5 | - |
5-methoxy-2,5-dioxopentanoic acid | pH 7.2, 30°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
Glutaramate | pH 7.2, 30°C | Mus musculus | |
6.9 | - |
Glutaramate | pH 8.5, 30°C | Mus musculus | |
8.7 | - |
succinamate | pH 7.2, 30°C | Mus musculus | |
20 | - |
succinamate | pH 8.5, 30°C | Mus musculus | |
51.2 | - |
2-oxo-succinamate | pH 8.5, 30°C | Mus musculus | |
89.7 | - |
2-oxo-glutaramate | pH 8.5, 30°C | Mus musculus | |
385 | - |
2-oxo-succinamate | pH 7.2, 30°C | Mus musculus | |
11200 | - |
5-methoxy-2,5-dioxopentanoic acid | pH 7.2, 30°C | Mus musculus |