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Literature summary for 3.5.1.2 extracted from

  • Jaisson, S.; Veiga-da-Cunha, M.; Van Schaftingen, E.
    Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2 (2009), Biochimie, 91, 1066-1071.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mouse nitrilase 2 is expressed as a His-tagged fusion protein in Escherichia coli and in HEK-293T cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
2-oxo-succinamate pH 7.2, 30°C Mus musculus
0.012
-
5-methoxy-2,5-dioxopentanoic acid pH 7.2, 30°C Mus musculus
0.017
-
2-oxo-succinamate pH 8.5, 30°C Mus musculus
0.14
-
succinamate pH 8.5, 30°C Mus musculus
0.195
-
2-oxo-glutaramate pH 8.5, 30°C Mus musculus
0.23
-
succinamate pH 7.2, 30°C Mus musculus
1.27
-
Glutaramate pH 8.5, 30°C Mus musculus
1.48
-
Glutaramate pH 7.2, 30°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-glutaramate + H2O
-
Mus musculus ?
-
?
2-oxo-succinamate + H2O
-
Mus musculus ?
-
?
5-methoxy-2,5-dioxopentanoic acid + H2O
-
Mus musculus methanol + 2-oxoglutaric acid
-
?
glutaramate + H2O
-
Mus musculus ?
-
?
succinamate + H2O
-
Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
Nit 2
-
Mus musculus
nitrilase 2
-
Mus musculus
omega-amidase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.87
-
2-oxo-succinamate pH 8.5, 30°C Mus musculus
1.15
-
2-oxo-succinamate pH 7.2, 30°C Mus musculus
2
-
succinamate pH 7.2, 30°C Mus musculus
2.8
-
succinamate pH 8.5, 30°C Mus musculus
4.1
-
Glutaramate pH 7.2, 30°C Mus musculus
8.8
-
Glutaramate pH 8.5, 30°C Mus musculus
17.5
-
2-oxo-glutaramate pH 8.5, 30°C Mus musculus
134.5
-
5-methoxy-2,5-dioxopentanoic acid pH 7.2, 30°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.8
-
Glutaramate pH 7.2, 30°C Mus musculus
6.9
-
Glutaramate pH 8.5, 30°C Mus musculus
8.7
-
succinamate pH 7.2, 30°C Mus musculus
20
-
succinamate pH 8.5, 30°C Mus musculus
51.2
-
2-oxo-succinamate pH 8.5, 30°C Mus musculus
89.7
-
2-oxo-glutaramate pH 8.5, 30°C Mus musculus
385
-
2-oxo-succinamate pH 7.2, 30°C Mus musculus
11200
-
5-methoxy-2,5-dioxopentanoic acid pH 7.2, 30°C Mus musculus