Any feedback?
Literature summary for 3.5.1.2 extracted from
- Glutaminase: a multifaceted protein not only involved in generating glutamate (2006), Neurochem. Int., 48, 465-471.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
the L gene, encoding the liver-type isozyme, is cloned from a brain library and is located on chromosome 12, while the kidney-type isozy| Homo sapiens |
|
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E599A | site-directed mutagenesis | Homo sapiens |
| M601A | site-directed mutagenesis | Homo sapiens |
| S600A | site-directed mutagenesis | Homo sapiens |
| V602A | site-directed mutagenesis | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-glutamate | strong product inhibition of isozyme KGA, no inhibition of isozyme LGA | Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | of neutrophils, isozyme LGA | Homo sapiens | 9986 | - |
| extracellular | isozyme LGA is secreted from the plasma membrane/cell surface of neutrophils | Homo sapiens | - |
- |
| mitochondrion | isozyme LGA | Homo sapiens | 5739 | - |
| mitochondrion | isozyme LGA | Rattus norvegicus | 5739 | - |
| additional information | analysis of subcellular targeting sequences and motifs of isozyme LGA | Homo sapiens | - |
- |
| nucleus | isozyme LGA, in brain | Homo sapiens | 5634 | - |
| nucleus | isozyme LGA, in brain | Rattus norvegicus | 5634 | - |
| plasma membrane | of neutrophils, isozyme LGA | Homo sapiens | 5886 | - |
| secretory granule | isozyme LGA | Homo sapiens | 30141 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | phosphate-activated glutaminase isozyme | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + H2O | Rattus norvegicus | the enzyme plays a key role in energy and nitrogen metabolism | L-glutamate + NH3 | - |
? | |
| L-glutamine + H2O | Homo sapiens | the enzyme plays a key role in energy and nitrogen metabolism, glutamate is a major excitatory neurotransmitter in neurons, especially in the cerebral cortex | L-glutamate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
two isozymes, glutaminase K and L, i.e. LGA and KGA, or liver-type isozyme and kidney-type isozyme | - |
| no activity in Gallus gallus | - |
no liver-type isozyme, LGA, activity in the brain | - |
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| astrocyte | isozymes LGA and KGA | Homo sapiens | - |
| brain | isozymes LGA and KGA | Rattus norvegicus | - |
| brain | isozymes LGA and KGA, mainly astrocytes, cerebral cortex, and neurons | Homo sapiens | - |
| breast cancer cell | - |
Homo sapiens | - |
| cerebral cortex | isozymes LGA and KGA | Homo sapiens | - |
| colonic cancer cell | splicing variant isozyme GAC | Homo sapiens | - |
| kidney | isozyme KGA | Homo sapiens | - |
| kidney | isozyme KGA and splicing variant isozyme GAC | Rattus norvegicus | - |
| liver | isozyme LGA is specific for liver, and isozyme KGA is expressed in fetal liver | Rattus norvegicus | - |
| liver | isozymes LGA and KGA | Homo sapiens | - |
| lymphocyte | isozyme KGA | Rattus norvegicus | - |
| additional information | isozyme expression patterns | Rattus norvegicus | - |
| additional information | isozyme tissue-specific expression patterns, the enzyme is overexpressed in a variety of tumours and malignancies, overview | Homo sapiens | - |
| neuron | isozymes LGA and KGA | Homo sapiens | - |
| neutrophil | isozyme LGA is secreted from the plasma membrane | Homo sapiens | - |
| pancreas | isozymes LGA and KGA | Rattus norvegicus | - |
| small intestine | isozyme KGA | Rattus norvegicus | - |
| small intestine | isozymes LGA and KGA | Homo sapiens | - |
| spinal cord | low expression level of isozymes LGA and KGA | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + H2O | - |
Homo sapiens | L-glutamate + NH3 | - |
? | |
| L-glutamine + H2O | - |
Rattus norvegicus | L-glutamate + NH3 | - |
? | |
| L-glutamine + H2O | the enzyme plays a key role in energy and nitrogen metabolism | Rattus norvegicus | L-glutamate + NH3 | - |
? | |
| L-glutamine + H2O | the enzyme plays a key role in energy and nitrogen metabolism, glutamate is a major excitatory neurotransmitter in neurons, especially in the cerebral cortex | Homo sapiens | L-glutamate + NH3 | - |
? | |
| additional information | multifunctional isozyme LGA interacts with PDZ domain proteins, e.g. alpha-1-syntrophin or glutaminase-interacting protein GIP, via specific short sequence motifs, interaction mechanism, overview | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GA | - |
Homo sapiens |
| GA | - |
Rattus norvegicus |
| GAC | - |
Homo sapiens |
| GAC | - |
Rattus norvegicus |
| glutaminase K | - |
Homo sapiens |
| glutaminase K | - |
Rattus norvegicus |
| glutaminase L | - |
Homo sapiens |
| glutaminase L | - |
Rattus norvegicus |
| KAG | - |
Homo sapiens |
| KAG | - |
Rattus norvegicus |
| LAG | - |
Homo sapiens |
| LAG | - |
Rattus norvegicus |
| phosphate-activated glutaminase | - |
Homo sapiens |
| phosphate-activated glutaminase | - |
Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
