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Literature summary for 3.5.1.2 extracted from

  • Wakayama, M.; Yamagata, T.; Kamemura, A.; Bootim, N.; Yano, S.; Tachiki, T.; Yoshimune, K.; Moriguchi, M.
    Characterization of salt-tolerant glutaminase from Stenotrophomonas maltophilia NYW-81 and its application in Japanese soy sauce fermentation (2005), J. Ind. Microbiol. Biotechnol., 32, 383-390.
    View publication on PubMed
Search for more literature for 3.5.1.2

Application

Application Comment Organism
nutrition the enzyme is applicated in Japanese soy sauce fermentation Stenotrophomonas maltophilia

Inhibitors

Inhibitors Comment Organism Structure
CoCl2 14% inhibition at 1 mM Stenotrophomonas maltophilia
CuCl2 20% inhibition at 1 mM, 17% at 0.1 mM Stenotrophomonas maltophilia
EDTA 10% inhibition at 1 mM Stenotrophomonas maltophilia
MgCl2 20% inhibition at 1 mM, 12% at 0.1 mM Stenotrophomonas maltophilia
MnCl2 22% inhibition at 1 mM, 13% at 0.1 mM Stenotrophomonas maltophilia
ZnCl2 7% inhibition at 1 mM Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 25% activation at 1 mM Stenotrophomonas maltophilia
additional information the enzyme is not affected by NiCl2 at 1 mM Stenotrophomonas maltophilia
NaCl the enzyme is salt-tolerant, over 86% of maximal activity at 16% NaCl, no effect at 1 mM Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 36000, SDS-PAGE Stenotrophomonas maltophilia
41000
-
 gel filtration Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + H2O Stenotrophomonas maltophilia
-
 L-glutamate + NH3
-
 ?
L-glutamine + H2O Stenotrophomonas maltophilia NYW-81
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-
Stenotrophomonas maltophilia NYW-81
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 250fold from strain NYW-81 to homogeneity by two steps of anion exchange chromatography, hydrophobic interaction and hydroxylapatite chromatography, and gel filtration Stenotrophomonas maltophilia

Source Tissue

Source Tissue Comment Organism Textmining
additional information strain NYW-81, Koji medium, effects of carbon and nitrogen sources for cell growth on the production of enzyme, overview Stenotrophomonas maltophilia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
325
-
 purified enzyme Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-asparagine + H2O 67% of the activity with L-glutamine Stenotrophomonas maltophilia D-aspartate + NH3
-
 ?
D-asparagine + H2O 67% of the activity with L-glutamine Stenotrophomonas maltophilia NYW-81 D-aspartate + NH3
-
 ?
D-glutamine + H2O 75% of the activity with L-glutamine Stenotrophomonas maltophilia D-glutamate + NH3
-
 ?
D-glutamine + H2O 75% of the activity with L-glutamine Stenotrophomonas maltophilia NYW-81 D-glutamate + NH3
-
 ?
L-asparagine + H2O 74% of the activity with L-glutamine Stenotrophomonas maltophilia L-aspartate + NH3
-
 ?
L-asparagine + H2O 74% of the activity with L-glutamine Stenotrophomonas maltophilia NYW-81 L-aspartate + NH3
-
 ?
L-glutamine + H2O
-
 Stenotrophomonas maltophilia L-glutamate + NH3
-
 ?
L-glutamine + H2O best substrate Stenotrophomonas maltophilia L-glutamate + NH3
-
 ?
L-glutamine + H2O
-
 Stenotrophomonas maltophilia NYW-81 L-glutamate + NH3
-
 ?
L-glutamine + H2O best substrate Stenotrophomonas maltophilia NYW-81 L-glutamate + NH3
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Stenotrophomonas maltophilia

Synonyms

Synonyms Comment Organism
salt-tolerant glutaminase
-
 Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Stenotrophomonas maltophilia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, 70% remaining activity, purified enzyme Stenotrophomonas maltophilia
70
-
 10 min, 50% remaining activity, purified enzyme Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Stenotrophomonas maltophilia

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 most stable at pH 8.0, 60-80% of maximal activity at pH 5.0-10.0, purified enzyme Stenotrophomonas maltophilia