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Literature summary for 3.5.1.2 extracted from
- Isolation of a novel, phosphate-activated glutaminase from Bacillus pasteurii (2002), FEMS Microbiol. Lett., 206, 63-67.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity is not significantly impaired with 2-oxoglutarate, pyruvate, succinate and citrate, no inhibition with L-glutamate, NH4+ and L-aspartate | Sporosarcina pasteurii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Sporosarcina pasteurii | |
| 9.5 | - |
L-glutamine | pH 9.0, at 37ºC, H2O as co-substrate | Sporosarcina pasteurii |  |
| 15 | - |
gamma-glutamyl hydroxamate | pH 9.0, at 37ºC, NH2OH as co-substrate | Sporosarcina pasteurii | |
| 17 | - |
L-glutamine | pH 9.0, at 37ºC, NH2OH as co-substrate | Sporosarcina pasteurii | |
| 24 | - |
L-Asn | pH 9.0, at 37ºC, H2O as co-substrate | Sporosarcina pasteurii | |
| 83 | - |
D-glutamine | pH 9.0, at 37ºC, H2O as co-substrate | Sporosarcina pasteurii | |
| 500 | - |
L-Glutamic acid | pH 9.0, at 37ºC, NH2OH as co-substrate | Sporosarcina pasteurii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Sporosarcina pasteurii | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | 50 mM, 800% of the relative activity without addition | Sporosarcina pasteurii | |
| additional information | no effect with NaCl, no significant alteration with KCl, MgCl2, MnCl2, CaCl2 and NH4Cl | Sporosarcina pasteurii | |
| phosphate | 50 mM, 900% of the relative activity without addition | Sporosarcina pasteurii | |
| sulfate | 50 mM, 700% of the relative activity without addition | Sporosarcina pasteurii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Sporosarcina pasteurii |
| 110000 | - |
PAGE under nondenaturing conditions | Sporosarcina pasteurii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + H2O | Sporosarcina pasteurii | glutamine catabolism | L-glutamate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sporosarcina pasteurii | - |
ATCC 11859 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | - |
Sporosarcina pasteurii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by two-step ammonium sulfate precipitation, hydrophobic interaction chromatography on phenyl-Sepharose, anion exchange chromatography on DEAE-cellulose, hydrophobic interaction chromatography on phenyl-Superose and anion exchange chromatography using Mono Q, 762fold | Sporosarcina pasteurii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Sporosarcina pasteurii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 234 | - |
- |
Sporosarcina pasteurii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Asn + H2O | less 0.1% of the activity with L-glutamine and H2O as co-substrate | Sporosarcina pasteurii | D-aspartate + NH3 | - |
? | |
| D-glutamine + H2O | 8.1% of the activity with L-glutamine and H2O as co-substrate | Sporosarcina pasteurii | D-glutamate + NH3 | - |
? | |
| gamma-glutamyl hydroxamate + NH2OH | 36.3% of the activity with L-glutamine and H2O as co-substrate | Sporosarcina pasteurii | ? | - |
? | |
| L-Asn + H2O | 20.6% of the activity with L-glutamine and H2O as co-substrate | Sporosarcina pasteurii | L-aspartate + NH3 | - |
? | |
| L-glutamic acid + NH2OH | 0.9% of the activity with L-glutamine and H2O as co-substrate | Sporosarcina pasteurii | gamma-glutamyl hydroxamate | - |
? | |
| L-glutamine + H2O | - |
Sporosarcina pasteurii | L-glutamate + NH3 | - |
? | |
| L-glutamine + H2O | glutamine catabolism | Sporosarcina pasteurii | L-glutamate + NH3 | - |
? | |
| L-glutamine + NH2OH | 10.3% of the activity with L-glutamine and H2O as co-substrate | Sporosarcina pasteurii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 55000, SDS-PAGE | Sporosarcina pasteurii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-glutamine amidohydrolase | - |
Sporosarcina pasteurii |
| phosphate activated glutaminase | - |
Sporosarcina pasteurii |
| phosphate-activated L-glutamine amidohydrolase | - |
Sporosarcina pasteurii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Sporosarcina pasteurii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Sporosarcina pasteurii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sporosarcina pasteurii | isoelectric focusing | - |
4.9 |
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Release 2023.1 (January 2023)
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