Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | CuCl2, inhibition of glutaminase II | Micrococcus luteus | |
EDTA | glutaminase I and II | Micrococcus luteus | |
FeCl3 | glutaminase II | Micrococcus luteus | |
Na2SO4 | glutaminase I and II | Micrococcus luteus | |
PCMB | inhibition of glutaminase I but not glutaminase II | Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
L-Gln | glutaminase I | Micrococcus luteus | |
6.5 | - |
L-Gln | glutaminase II | Micrococcus luteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 8-16%, maximal activity of glutaminase I, no effect of glutaminase II | Micrococcus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, glutaminase I, SDS-PAGE | Micrococcus luteus |
86000 | - |
glutaminase I and II, gel filtration | Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Gln + H2O | Micrococcus luteus | constitutive enzyme | L-Glu + NH3 | - |
? | |
L-Gln + H2O | Micrococcus luteus K-3 | constitutive enzyme | L-Glu + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
glutaminase I and II | - |
Micrococcus luteus K-3 | - |
glutaminase I and II | - |
Purification (Comment) | Organism |
---|---|
purification of glutaminase I, partial purification of glutaminase II | Micrococcus luteus |
Storage Stability | Organism |
---|---|
0-5°C, pH 7.2, 10 mM Tris-HCl, 10% glycerol, 10 mM MgCl2, glutaminase I and II, stable for 1 month | Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gln + H2O | - |
Micrococcus luteus | Glu + NH3 | - |
? | |
Gln + H2O | - |
Micrococcus luteus K-3 | Glu + NH3 | - |
? | |
L-Gln + H2O | - |
Micrococcus luteus | L-Glu + NH3 | - |
? | |
L-Gln + H2O | constitutive enzyme | Micrococcus luteus | L-Glu + NH3 | - |
? | |
L-Gln + H2O | - |
Micrococcus luteus K-3 | L-Glu + NH3 | - |
? | |
L-Gln + H2O | constitutive enzyme | Micrococcus luteus K-3 | L-Glu + NH3 | - |
? | |
L-Gln + hydroxylamine | no activity | Micrococcus luteus | L-gamma-glutamyl hydroxamate + NH3 | - |
? | |
L-Gln + hydroxylamine | no activity | Micrococcus luteus K-3 | L-gamma-glutamyl hydroxamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, glutaminase I, SDS-PAGE | Micrococcus luteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
glutaminase I and II | Micrococcus luteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
10% NaCl enhances thermal stability of glutaminase I | Micrococcus luteus |
30 | - |
pH 7.5, 10 min, stable in absence of NaCl | Micrococcus luteus |
40 | - |
pH 7.5, 10 min, 10% NaCl, glutaminase I and II stable up to | Micrococcus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
glutaminase I | Micrococcus luteus |
8.5 | - |
glutaminase II | Micrococcus luteus |