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Literature summary for 3.5.1.2 extracted from

  • Moriguchi, M.; Sakai, K.; Tateyama, R.; Furuta, Y.; Wakayama, M.
    Isolation and characterization of salt-tolerant glutaminases from marine Micrococcus luteus K-3 (1994), J. Ferment. Bioeng., 77, 621-625.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ CuCl2, inhibition of glutaminase II Micrococcus luteus
EDTA glutaminase I and II Micrococcus luteus
FeCl3 glutaminase II Micrococcus luteus
Na2SO4 glutaminase I and II Micrococcus luteus
PCMB inhibition of glutaminase I but not glutaminase II Micrococcus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4
-
L-Gln glutaminase I Micrococcus luteus
6.5
-
L-Gln glutaminase II Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 8-16%, maximal activity of glutaminase I, no effect of glutaminase II Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, glutaminase I, SDS-PAGE Micrococcus luteus
86000
-
glutaminase I and II, gel filtration Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Gln + H2O Micrococcus luteus constitutive enzyme L-Glu + NH3
-
?
L-Gln + H2O Micrococcus luteus K-3 constitutive enzyme L-Glu + NH3
-
?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
glutaminase I and II
-
Micrococcus luteus K-3
-
glutaminase I and II
-

Purification (Commentary)

Purification (Comment) Organism
purification of glutaminase I, partial purification of glutaminase II Micrococcus luteus

Storage Stability

Storage Stability Organism
0-5°C, pH 7.2, 10 mM Tris-HCl, 10% glycerol, 10 mM MgCl2, glutaminase I and II, stable for 1 month Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gln + H2O
-
Micrococcus luteus Glu + NH3
-
?
Gln + H2O
-
Micrococcus luteus K-3 Glu + NH3
-
?
L-Gln + H2O
-
Micrococcus luteus L-Glu + NH3
-
?
L-Gln + H2O constitutive enzyme Micrococcus luteus L-Glu + NH3
-
?
L-Gln + H2O
-
Micrococcus luteus K-3 L-Glu + NH3
-
?
L-Gln + H2O constitutive enzyme Micrococcus luteus K-3 L-Glu + NH3
-
?
L-Gln + hydroxylamine no activity Micrococcus luteus L-gamma-glutamyl hydroxamate + NH3
-
?
L-Gln + hydroxylamine no activity Micrococcus luteus K-3 L-gamma-glutamyl hydroxamate + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, glutaminase I, SDS-PAGE Micrococcus luteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
glutaminase I and II Micrococcus luteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
10% NaCl enhances thermal stability of glutaminase I Micrococcus luteus
30
-
pH 7.5, 10 min, stable in absence of NaCl Micrococcus luteus
40
-
pH 7.5, 10 min, 10% NaCl, glutaminase I and II stable up to Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
glutaminase I Micrococcus luteus
8.5
-
glutaminase II Micrococcus luteus