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Literature summary for 3.5.1.2 extracted from

  • Prusiner, S.; Davis, J.N.; Stadtman, E.R.
    Regulation of glutaminase B in Escherichia coli. I. Purification, properties, and cold lability (1976), J. Biol. Chem., 251, 3447-3456.
    View publication on PubMed

General Stability

General Stability Organism
significant loss of activity upon freezing at -20°C and -80°C in presence and absence of 20% glycerol Escherichia coli
sodium borate is the most effective stabilizing agent against cold inactivation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
glutaminase B, gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutaminase B Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
4°C, in presence of sodium borate, stable for 5 days Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gln + H2O
-
Escherichia coli Glu + NH3
-
?
L-Gln + hydroxylamine
-
Escherichia coli L-gamma-glutamyl hydroxamate + NH3
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
low level of activity after partial purification at 4°C in absence of stabilizing ligands, warming at 24°C results in 2fold to 5fold increase in activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1 9 hydrolysis of Gln, glutaminase B Escherichia coli
8.3 9.2 formation of gamma-glutamyl hydroxamate from L-Gln and NH2OH Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 11 pH 6.0: about 55% of maximal activity, pH 11.0: about 55% of maximal activity, hydrolysis of Gln Escherichia coli