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Literature summary for 3.5.1.2 extracted from
- Comparison of the phosphate-dependent glutaminase obtained from rat brain and kidney (1985), Biochem. J., 229, 399-408.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | dependent on | Rattus norvegicus |  |
| phosphate | 100 mM, maximal activation and dimerization of the enzyme | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glutamate | competitive with respect to Gln | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
Gln | in presence of 150 mM phosphate | Rattus norvegicus | |
| 14 | - |
Gln | in presence of 10 mM phosphate | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | synaptosomal and non-synaptosomal | Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000 + x * 68000, protein bands in the ratio 4:1, SDS-PAGE | Rattus norvegicus |
| 68000 | - |
x * 65000 + x * 68000, protein bands in the ratio 4:1, SDS-PAGE | Rattus norvegicus |
| 126000 | - |
gel filtration | Rattus norvegicus |
| 249000 | - |
gel filtration after dimerization with phosphate | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 345 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gln + H2O | - |
Rattus norvegicus | Glu + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000 + x * 68000, protein bands in the ratio 4:1, SDS-PAGE | Rattus norvegicus |
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Release 2023.1 (January 2023)
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