Crystallization (Comment) | Organism |
---|---|
- |
Acinetobacter glutaminasificans |
General Stability | Organism |
---|---|
freezing and thawing produces a substantial loss of activity | Acinetobacter glutaminasificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | - |
Acinetobacter glutaminasificans | |
glutamate | no inhibition | Acinetobacter glutaminasificans | |
methylene blue | 0.005%, irreversible inactivation | Acinetobacter glutaminasificans | |
Rose bengal | 0.01%, irreversible inactivation | Acinetobacter glutaminasificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
L-Asn | - |
Acinetobacter glutaminasificans | |
5.8 | - |
L-Gln | - |
Acinetobacter glutaminasificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000 | Acinetobacter glutaminasificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter glutaminasificans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter glutaminasificans |
Storage Stability | Organism |
---|---|
5°C, enzyme preparation as a lyophilized powder, indefinitely stable | Acinetobacter glutaminasificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-cyanoalanine + H2O | 0.2% of the activity with L-Gln | Acinetobacter glutaminasificans | ? | - |
? | |
D-Asn + H2O | 26% of the activity with L-Gln | Acinetobacter glutaminasificans | D-Asp + NH3 | - |
? | |
D-Gln + H2O | 35% of the activity with L-Gln | Acinetobacter glutaminasificans | D-Glu + NH3 | - |
? | |
Gln + H2O | - |
Acinetobacter glutaminasificans | Glu + NH3 | - |
? | |
L-Asn + H2O | 77% of the activity with L-Gln | Acinetobacter glutaminasificans | L-Asp + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000 | Acinetobacter glutaminasificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
hydrolysis of L-Asn and L-Gln | Acinetobacter glutaminasificans |