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Literature summary for 3.5.1.19 extracted from

  • Stekhanova, T.N.; Bezsudnova, E.Y.; Mardanov, A.V.; Osipov, E.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.
    Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics (2014), Biochemistry, 79, 54-61.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2 mM, 2fold increase in activity Acidilobus saccharovorans
dithiothreitol 2 mM, 2.1fold increase in activity Acidilobus saccharovorans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Acidilobus saccharovorans

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, 57% loss of activity Acidilobus saccharovorans
Fe3+ 1 mM, 94% inhibition Acidilobus saccharovorans
NaCl 500 mM, 12% inhibition Acidilobus saccharovorans
Zn2+ 5 mM, complete inactivation Acidilobus saccharovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
nicotinamide pH and temperature not specified in the publication Acidilobus saccharovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains Fe2+ in the active site Acidilobus saccharovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44600
-
-
Acidilobus saccharovorans

Organism

Organism UniProt Comment Textmining
Acidilobus saccharovorans D9Q1R5
-
-
Acidilobus saccharovorans DSM 16705 D9Q1R5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide + H2O the enzyme also catalyzes the hydrolysis of pyrazinamide Acidilobus saccharovorans nicotinate + NH3
-
?
nicotinamide + H2O the enzyme also catalyzes the hydrolysis of pyrazinamide Acidilobus saccharovorans DSM 16705 nicotinate + NH3
-
?

Subunits

Subunits Comment Organism
homodimer
-
Acidilobus saccharovorans

Synonyms

Synonyms Comment Organism
ASAC_0847 locus name Acidilobus saccharovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Acidilobus saccharovorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 98 65°C: about 50% of maximal activity, 98°C: about 80% of maximal activity Acidilobus saccharovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
t1/2: 180 min Acidilobus saccharovorans
80
-
t1/2: 35 min Acidilobus saccharovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
76.9
-
nicotinamide pH and temperature not specified in the publication Acidilobus saccharovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Acidilobus saccharovorans

General Information

General Information Comment Organism
physiological function the enzyme is involved in the maintenance of NAD+ homeostasis and in the NAD+ salvage pathway of most prokaryotes Acidilobus saccharovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
427
-
nicotinamide pH and temperature not specified in the publication Acidilobus saccharovorans