Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2 mM, 2fold increase in activity | Acidilobus saccharovorans | |
dithiothreitol | 2 mM, 2.1fold increase in activity | Acidilobus saccharovorans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Acidilobus saccharovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, 57% loss of activity | Acidilobus saccharovorans | |
Fe3+ | 1 mM, 94% inhibition | Acidilobus saccharovorans | |
NaCl | 500 mM, 12% inhibition | Acidilobus saccharovorans | |
Zn2+ | 5 mM, complete inactivation | Acidilobus saccharovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
nicotinamide | pH and temperature not specified in the publication | Acidilobus saccharovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains Fe2+ in the active site | Acidilobus saccharovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44600 | - |
- |
Acidilobus saccharovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidilobus saccharovorans | D9Q1R5 | - |
- |
Acidilobus saccharovorans DSM 16705 | D9Q1R5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | the enzyme also catalyzes the hydrolysis of pyrazinamide | Acidilobus saccharovorans | nicotinate + NH3 | - |
? | |
nicotinamide + H2O | the enzyme also catalyzes the hydrolysis of pyrazinamide | Acidilobus saccharovorans DSM 16705 | nicotinate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Acidilobus saccharovorans |
Synonyms | Comment | Organism |
---|---|---|
ASAC_0847 | locus name | Acidilobus saccharovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Acidilobus saccharovorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 98 | 65°C: about 50% of maximal activity, 98°C: about 80% of maximal activity | Acidilobus saccharovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
t1/2: 180 min | Acidilobus saccharovorans |
80 | - |
t1/2: 35 min | Acidilobus saccharovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
76.9 | - |
nicotinamide | pH and temperature not specified in the publication | Acidilobus saccharovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Acidilobus saccharovorans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the maintenance of NAD+ homeostasis and in the NAD+ salvage pathway of most prokaryotes | Acidilobus saccharovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
427 | - |
nicotinamide | pH and temperature not specified in the publication | Acidilobus saccharovorans |