Any feedback?
Literature summary for 3.5.1.19 extracted from
- Kinetics and inhibition of nicotinamidase from Mycobacterium tuberculosis (2010), Biochemistry, 49, 9613-9619.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H57D | the mutant has reduced Mn2+ content and shows a 6fold and 38fold decrease in kcat value for nicotinamide and pyrazinamide, respectively | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-pyridine carboxaldehyde | competitive inhibitor | Mycobacterium tuberculosis |  |
| iodoacetamide | reversible, competitive inhibitor | Mycobacterium tuberculosis | |
| pyrazinecarbonitrile | irreversible inactivator | Mycobacterium tuberculosis | |
| pyridine-3-carbonitrile | reversible, competitive inhibitor | Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.014 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.016 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.3 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme contains 0.47 M of Fe2+ per mol of enzyme | Mycobacterium tuberculosis | |
| Mn2+ | the enzyme contains 0.44 M Mn2+ per mol of enzyme | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | Mycobacterium tuberculosis | - |
nicotinate + NH3 | - |
? | |
| nicotinamide + H2O | Mycobacterium tuberculosis H37Rv | - |
nicotinate + NH3 | - |
? | |
| pyrazinamide + H2O | Mycobacterium tuberculosis | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
| pyrazinamide + H2O | Mycobacterium tuberculosis H37Rv | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | - |
Mycobacterium tuberculosis | nicotinate + NH3 | - |
? | |
| nicotinamide + H2O | - |
Mycobacterium tuberculosis H37Rv | nicotinate + NH3 | - |
? | |
| pyrazinamide + H2O | - |
Mycobacterium tuberculosis | pyrazine-2-carboxylic acid + NH3 | - |
? | |
| pyrazinamide + H2O | - |
Mycobacterium tuberculosis H37Rv | pyrazine-2-carboxylic acid + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nicotinamidase/pyrazinamidase | - |
Mycobacterium tuberculosis |
| PncA | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.5 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 3.1 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 3.8 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
around pH 8.0 | Mycobacterium tuberculosis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00029 | - |
3-pyridine carboxaldehyde | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 0.1 | - |
pyridine-3-carbonitrile | Ki about 0.1 mM, wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the NAD+ salvage pathway | Mycobacterium tuberculosis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.2 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 13 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 37 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
| 220 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
