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Literature summary for 3.5.1.19 extracted from

  • Vrablik, T.L.; Huang, L.; Lange, S.E.; Hanna-Rose, W.
    Nicotinamidase modulation of NAD+ biosynthesis and nicotinamide levels separately affect reproductive development and cell survival in C. elegans (2009), Development, 136, 3637-3646.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
isolation of a pnc-1a promoter consisting of exon 1a and the intervening region between exon 1a and the adjacent gene, expression as GFP-fusion protein. Expression of His-tagged PNC proteins in Escherichia coli strain BL21(DE3) Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
additional information mutations in PNC-1 cause developmental and functional defects in the reproductive system. The development of the gonad is delayed, four uterine cells die by necrosis and the mutant animals are egg-laying defective. The temporal delay in gonad development results from depletion of the salvage pathway product NAD+, whereas the uv1 cell necrosis and egg-laying defects result from accumulation of the substrate nicotinamide, phenotype, overview. The bacterial food source partially compensates for pnc-1 reproductive defects. Although the secreted isoform is sufficient to rescue all phenotypes, the concentrations of NAD+ and nicotinamide in the cytoplasm and/or cellular organelles presumably elicit phenotypes Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted isoform of PNC-1 Caenorhabditis elegans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinamide + H2O Caenorhabditis elegans
-
nicotinate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide + H2O
-
Caenorhabditis elegans nicotinate + NH3
-
?

Synonyms

Synonyms Comment Organism
PNC-1
-
Caenorhabditis elegans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Caenorhabditis elegans

General Information

General Information Comment Organism
metabolism nicotinamide phosphoribosyltransferase, Nampt, the equivalent enzyme in nicotinamide recycling to NAD+ in vertebrates, can functionally substitute for PNC-1 Caenorhabditis elegans
additional information a correlation between the limited detected expression patterns of the PNC-1a and b promoters and the function of PNC-1 in development is not obvious Caenorhabditis elegans