Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | stress suppresses the inhibitory effect of nicotinamide through the induction of PNC1 expression | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
gene PNC1, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C167A | site-directed mutagenesis, inactive mutant, no rescue the inhibition of NAD+-dependent histone deacetylase, i.e. HDAC, activity of Sir2 by nicotinamide | Saccharomyces cerevisiae |
additional information | PNC1 overexpression suppresses the inhibitory effect of exogenously added nicotinamide on silencing, life span, and Hst1-mediated transcriptional repression | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | Saccharomyces cerevisiae | nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview | nicotinate + NH3 | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene PNC1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview | Saccharomyces cerevisiae | nicotinate + NH3 | - |
? | |
nicotinamide + H2O | Pnc1 stimulates Sir2, an NAD+-dependent histone deacetylase, and prevents the accumulation of nicotinamide | Saccharomyces cerevisiae | nicotinate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pnc1 | - |
Saccharomyces cerevisiae |