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Literature summary for 3.5.1.19 extracted from

  • Kgayama, T.; Ohe, T.
    Purification and properties of an aromatic amidase from Pseudomonas sp. GDI 211 (1990), Agric. Biol. Chem., 54, 2565-2571.
No PubMed abstract available
Search for more literature for 3.5.1.19

General Stability

General Stability Organism
enzyme is stable in buffer solution containing Co2+ and Ni2+ Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Pseudomonas sp.
Hg2+
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 nicotinamide
-
 Pseudomonas sp.
0.056
-
 Pyrazinamide
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enhances activity Pseudomonas sp.
Mn2+ enhances activity Pseudomonas sp.
Ni2+ enhances activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, SDS-PAGE Pseudomonas sp.
43000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinamide + H2O Pseudomonas sp.
-
 nicotinate + NH3
-
 ?
nicotinamide + H2O Pseudomonas sp. GDI 211
-
 nicotinate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. GDI 211
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37.6
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide + H2O
-
 Pseudomonas sp. nicotinate + NH3
-
 ?
nicotinamide + H2O 59.5% of the activity with pyrazinamide Pseudomonas sp. nicotinate + NH3
-
 ?
nicotinamide + H2O
-
 Pseudomonas sp. GDI 211 nicotinate + NH3
-
 ?
nicotinamide + H2O 59.5% of the activity with pyrazinamide Pseudomonas sp. GDI 211 nicotinate + NH3
-
 ?
pyrazinamide + H2O
-
 Pseudomonas sp. pyrazinoic acid + NH3
-
 ?
pyrazinamide + H2O
-
 Pseudomonas sp. GDI 211 pyrazinoic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 80 about 35% of maximal activity at 55°C and at 80°C Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 6.8, in presence of Co2+, 10 min, stable up to Pseudomonas sp.
70
-
 10 min, complete loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10.5 pH 5.0: about 70% of maximal activity, pH 10.5: about 75% of maximal activity Pseudomonas sp.