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Literature summary for 3.5.1.19 extracted from
- Purification, gene cloning, targeted knockout, overexpression, and biochemical characterization of the major pyrazinamidase from Mycobacterium smegmatis (1998), J. Bacteriol., 180, 5809-5814.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
nicotinamide | - |
Mycobacterium tuberculosis |  |
| 0.3 | - |
Pyrazinamide | - |
Mycobacterium tuberculosis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | - |
Mycolicibacterium smegmatis | nicotinate + NH3 | - |
? | |
| nicotinamide + H2O | - |
Mycobacterium tuberculosis | nicotinate + NH3 | - |
? | |
| pyrazinamide + H2O | - |
Mycolicibacterium smegmatis | pyrazinoic acid + NH3 | - |
? | |
| pyrazinamide + H2O | - |
Mycobacterium tuberculosis | pyrazinoic acid + NH3 | - |
? | |
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