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Literature summary for 3.5.1.19 extracted from
- Purification and characterization of a nicotinamide deamidase released into the growth medium of neuroblastoma in vitro (1980), Biochim. Biophys. Acta, 613, 191-202.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Serum albumin | 0.02%, 9% activation | Mus musculus |
General Stability
| General Stability | Organism |
|---|---|
| freezing and defreezing inactivates | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Mus musculus |  |
| Ag+ | - |
Mus musculus | |
| Cu2+ | - |
Mus musculus | |
| Cys | - |
Mus musculus | |
| dithiothreitol | - |
Mus musculus | |
| EDTA | 0.02 mM, 46% inhibition | Mus musculus | |
| Fe2+ | - |
Mus musculus | |
| Hg2+ | - |
Mus musculus | |
| NAD+ | competitive with respect to nicotinamide | Mus musculus | |
| sulfhydryl reagents | - |
Mus musculus | |
| Triton X-100 | 0.1%, 24% inhibition | Mus musculus | |
| Urea | 1 M, 20% inhibition | Mus musculus | |
| Zn2+ | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Mus musculus | |
| 0.006 | - |
nicotinamide | - |
Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Mus musculus | |
| Mg2+ | activation | Mus musculus | |
| Mn2+ | activation | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 65000 + 2 * 50000, SDS-PAGE | Mus musculus |
| 65000 | - |
2 * 65000 + 2 * 50000, SDS-PAGE | Mus musculus |
| 230000 | - |
gel electrophoresis | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | Mus musculus | enzyme is induced at high nicotinamide concentrations | nicotinate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 1.7% w/w mannose and 1.5% w/w N-acetylglucosamine | Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| C-1300 cell | C1300 neuroblastoma M1 clonal cell line | Mus musculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, stable for months | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | - |
Mus musculus | nicotinate + NH3 | - |
? | |
| nicotinamide + H2O | enzyme is induced at high nicotinamide concentrations | Mus musculus | nicotinate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 65000 + 2 * 50000, SDS-PAGE | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
half-life: 2.5 min | Mus musculus |
| 65 | - |
half-life: 1 min | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.5 | - |
Mus musculus |
| 7.2 | - |
- |
Mus musculus |
| 7.5 | - |
- |
Mus musculus |
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