Literature summary for 3.5.1.18 extracted from

Usha, V.; Lloyd, A.J.; Roper, D.I.; Dowson, C.G.; Kozlov, G.; Gehring, K.; Chauhan, S.; Imam, H.T.; Blindauer, C.A.; Besra, G.S.
Reconstruction of diaminopimelic acid biosynthesis allows characterisation of Mycobacterium tuberculosis N-succinyl-L,L-diaminopimelic acid desuccinylase (2016), Sci. Rep., 6, 23191 .

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli as an N-terminal hexahistidine ((His)6) tagged fusion containing one zinc ion per DapE monomer	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	-	Mycobacterium tuberculosis
2-thiopheneboronic acid	22.3% inhibition at 10 mM, non-competitive inhibitor	Mycobacterium tuberculosis
EDTA	-	Mycobacterium tuberculosis
L-captopril	99.96%, 26.67% and 23.34% inhibition at 10 mM, 3 mM, and 1 mM	Mycobacterium tuberculosis
L-Penicillamine	1 mM, 79.41% inhibition	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.03109	-	N-succinyl-LL-2,6-diaminoheptanedioate	pH 8.0, 37°C	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	untreated enzyme contains 0.8 Zn2+ per monomer and was enzymatically active	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O	Mycobacterium tuberculosis	-	succinate + LL-2,6-diaminoheptanedioate	-	?
N-succinyl-LL-2,6-diaminoheptanedioate + H2O	Mycobacterium tuberculosis H37Rv	-	succinate + LL-2,6-diaminoheptanedioate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WHS9	-	-
Mycobacterium tuberculosis H37Rv	P9WHS9	-	-

Purification (Commentary)

Purification (Comment)	Organism
N-terminal hexahistidine ((His)6) tagged fusion containing one zinc ion per DapE monomer	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O	-	Mycobacterium tuberculosis	succinate + LL-2,6-diaminoheptanedioate	-	?
N-succinyl-LL-2,6-diaminoheptanedioate + H2O	-	Mycobacterium tuberculosis H37Rv	succinate + LL-2,6-diaminoheptanedioate	-	?

Synonyms

Synonyms	Comment	Organism
DapE	-	Mycobacterium tuberculosis
Rv1202	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	42	at pH 8.0	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.85	-	N-succinyl-LL-2,6-diaminoheptanedioate	pH 8.0, 37°C	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
156	-	N-succinyl-LL-2,6-diaminoheptanedioate	pH 8.0, 37°C	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)