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Literature summary for 3.5.1.18 extracted from

  • Heath, T.K.; Lutz, M.R.; Reidl, C.T.; Guzman, E.R.; Herbert, C.A.; Nocek, B.P.; Holz, R.C.; Olsen, K.W.; Ballicora, M.A.; Becker, D.P.
    Practical spectrophotometric assay for the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase, a potential antibiotic target (2018), PLoS ONE, 13, e0196010 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Haemophilus influenzae

Inhibitors

Inhibitors Comment Organism Structure
D,L-2,6-diaminoheptanedioate competitive inhibitor Haemophilus influenzae
LL-2,6-Diaminoheptanedioate competitive inhibitor Haemophilus influenzae
succinate poor inhibitor Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 N-succinyl-LL-2,6-diaminoheptanedioate 25°C, pH 7.6, in the presence of Zn2+ Haemophilus influenzae
1.6
-
 N-succinyl-LL-2,6-diaminoheptanedioate 25°C, pH 7.6, in the presence of Co2+ Haemophilus influenzae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ metal-dependent enzyme (Zn2+ or Co2+). Determination of metal stoichiometry by ICP-AES indicates one tightly bound metal ion, while sequence homologies suggest the presence of two metal binding sites Haemophilus influenzae
Zn2+ metal-dependent enzyme (Zn2+ or Co2+). Determination of metal stoichiometry by ICP-AES indicates one tightly bound metal ion, while sequence homologies suggest the presence of two metal binding sites Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41350
-
 electrospray mass spectrometry Haemophilus influenzae
43000
-
 SDS-PAGE Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O Haemophilus influenzae
-
 succinate + LL-2,6-diaminoheptanedioate
-
 ?

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O
-
 Haemophilus influenzae succinate + LL-2,6-diaminoheptanedioate
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Haemophilus influenzae

Synonyms

Synonyms Comment Organism
DapE
-
 Haemophilus influenzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
200
-
 N-succinyl-LL-2,6-diaminoheptanedioate 25°C, pH 7.6, in the presence of Zn2+ Haemophilus influenzae
370
-
 N-succinyl-LL-2,6-diaminoheptanedioate 25°C, pH 7.6, in the presence of Co2+ Haemophilus influenzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.9 the maximal velocity is independent of pH above pH 7 when Zn2+ is used to activate the enzyme Haemophilus influenzae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.9 the maximal velocity is independent of pH above pH 7 when Zn2+ is used to activate the enzyme. Below pH 7, the maximum velocity decreases with an apparent slope of 1, indicating that protonation of a single ionizable group, exhibiting a pK value of 6.6 Haemophilus influenzae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
8
-
 LL-2,6-Diaminoheptanedioate 25°C, pH 7.6 Haemophilus influenzae
12
-
 D,L-2,6-diaminoheptanedioate 25°C, pH 7.6 Haemophilus influenzae