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Literature summary for 3.5.1.17 extracted from
- epsilon-lysine acylase from Achromobacter pestifer (1970), Methods Enzymol., 19, 756-762.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Achromobacter pestifer |  |
| citrate | slight inhibitory effect | Achromobacter pestifer | |
| diphosphate | marked inhibition | Achromobacter pestifer | |
| EDTA | slight inhibitory effect | Achromobacter pestifer | |
| Hg+ | - |
Achromobacter pestifer | |
| Hg2+ | - |
Achromobacter pestifer | |
| Monoiodoacetic acid | - |
Achromobacter pestifer | |
| omega-chloroacetophenone | - |
Achromobacter pestifer | |
| oxalate | marked inhibition | Achromobacter pestifer | |
| p-chloromercuribenzoate | inhibition is completely recovered by addition of cysteine | Achromobacter pestifer | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
N6-benzoyl-L-lysine | - |
Achromobacter pestifer | |
| 0.29 | - |
N6-chloroacetyl-L-lysine | - |
Achromobacter pestifer | |
| 0.5 | - |
N6-acetyl-L-lysine | - |
Achromobacter pestifer | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| epsilon-N-acyl-L-lysine + H2O | Gallus gallus | - |
? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | Rattus norvegicus | - |
? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | Sus scrofa | - |
? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | Pseudomonas sp. | - |
? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | Mushroom | - |
? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | Aspergillus oryzae | - |
? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | Achromobacter pestifer | - |
? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | Columba sp. | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter pestifer | - |
EA ATCC 23584 | - |
| Aspergillus oryzae | - |
No. 10, mould | - |
| Columba sp. | - |
pigeon | - |
| Gallus gallus | - |
chicken | - |
| Mushroom | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
| Rattus norvegicus | - |
rat | - |
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
- |
Sus scrofa |
- |
Pseudomonas sp. |
- |
Aspergillus oryzae |
- |
Achromobacter pestifer |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Gallus gallus | - |
| brain | - |
Rattus norvegicus | - |
| brain | - |
Sus scrofa | - |
| brain | - |
Columba sp. | - |
| heart | - |
Gallus gallus | - |
| heart | - |
Rattus norvegicus | - |
| heart | - |
Sus scrofa | - |
| heart | - |
Columba sp. | - |
| kidney | richest source | Gallus gallus | - |
| kidney | richest source | Rattus norvegicus | - |
| kidney | richest source | Sus scrofa | - |
| kidney | richest source | Columba sp. | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Columba sp. | - |
| pancreas | - |
Gallus gallus | - |
| pancreas | - |
Rattus norvegicus | - |
| pancreas | - |
Sus scrofa | - |
| pancreas | - |
Columba sp. | - |
| spleen | - |
Gallus gallus | - |
| spleen | - |
Rattus norvegicus | - |
| spleen | - |
Sus scrofa | - |
| spleen | - |
Columba sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.5 | - |
homogenous protein, vertical zone electrophoresis, purification 60.4fold | Achromobacter pestifer |
| 240 | - |
cell-free extract, purification 1fold | Achromobacter pestifer |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 60% ammonium sulfate, can be stored several months without decrease of activity | Achromobacter pestifer |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| epsilon-N-acyl-L-lysine + H2O | - |
Gallus gallus | ? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | - |
Sus scrofa | ? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | - |
Mushroom | ? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | - |
Achromobacter pestifer | ? | - |
? | |
| epsilon-N-acyl-L-lysine + H2O | - |
Columba sp. | ? | - |
? | |
| additional information | enzyme is inducibly formed by N6-benzoyl-L-, -D-, or -DL-lysine, no optical specificity on hydrolysis of N6-acyl-DL-lysine, the differences indicate that the epsilon-lysine acylases from animal, mould and bacterial sources are different enzymes | Aspergillus oryzae | ? | - |
? | |
| N6-acetyl-L-lysine + H2O | - |
Gallus gallus | L-lysine + acetate | - |
? | |
| N6-acetyl-L-lysine + H2O | - |
Rattus norvegicus | L-lysine + acetate | - |
? | |
| N6-acetyl-L-lysine + H2O | - |
Sus scrofa | L-lysine + acetate | - |
? | |
| N6-acetyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + acetate | - |
? | |
| N6-acyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + carboxylic acid | - |
? | |
| N6-alpha-naphthoyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + 1-naphthol | - |
? | |
| N6-benzoyl-L-lysine + H2O | - |
Gallus gallus | L-lysine + benzoate | - |
? | |
| N6-benzoyl-L-lysine + H2O | - |
Rattus norvegicus | L-lysine + benzoate | - |
? | |
| N6-benzoyl-L-lysine + H2O | - |
Sus scrofa | L-lysine + benzoate | - |
? | |
| N6-benzoyl-L-lysine + H2O | - |
Pseudomonas sp. | L-lysine + benzoate | - |
? | |
| N6-benzoyl-L-lysine + H2O | - |
Aspergillus oryzae | L-lysine + benzoate | - |
? | |
| N6-benzoyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + benzoate | - |
? | |
| N6-benzoyl-L-lysine + H2O | - |
Columba sp. | L-lysine + benzoate | - |
? | |
| N6-butyryl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + butanoate | - |
? | |
| N6-caproyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + n-hexanoate | - |
? | |
| N6-caprylyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + caprylic acid | - |
? | |
| N6-carbobenzoxy-L-lysine + H2O | - |
Gallus gallus | L-lysine + benzoate | - |
? | |
| N6-carbobenzoxy-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + benzoate | - |
? | |
| N6-carbobenzoxy-L-lysine + H2O | - |
Columba sp. | L-lysine + benzoate | - |
? | |
| N6-chloroacetyl-L-lysine + H2O | - |
Gallus gallus | L-lysine + chloroacetate | - |
? | |
| N6-chloroacetyl-L-lysine + H2O | - |
Rattus norvegicus | L-lysine + chloroacetate | - |
? | |
| N6-chloroacetyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + chloroacetate | - |
? | |
| N6-chloroacetyl-L-lysine + H2O | - |
Columba sp. | L-lysine + chloroacetate | - |
? | |
| N6-cyclohexanecarboxyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + cyclohexanecarboxylate | - |
? | |
| N6-dichloroacetyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + dichloroacetate | - |
? | |
| N6-glycyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + glycine | - |
? | |
| N6-iodoacetyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + iodoacetate | - |
? | |
| N6-isobutyryl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + isobutyrate | - |
? | |
| N6-m-nitrobenzoyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + 3-nitrobenzoate | - |
? | |
| N6-metacrylyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + ? | - |
? | |
| N6-p-nitrobenzoyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + 4-nitrobenzoate | - |
? | |
| N6-phenylacetyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + phenylacetate | - |
? | |
| N6-trichloroacetyl-L-lysine + H2O | - |
Achromobacter pestifer | L-lysine + trichloroacetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| epsilon-lysine acylase | - |
Gallus gallus |
| epsilon-lysine acylase | - |
Rattus norvegicus |
| epsilon-lysine acylase | - |
Achromobacter pestifer |
| epsilon-lysine acylase | - |
Columba sp. |
| epsilon-N-acyl-L-lysine amidohydrolase | - |
Achromobacter pestifer |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 5.2 | - |
Achromobacter pestifer |
| 6 | - |
- |
Pseudomonas sp. |
| 7 | - |
- |
Rattus norvegicus |
| 8.2 | 8.4 | - |
Aspergillus oryzae |
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Release 2023.1 (January 2023)
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