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Literature summary for 3.5.1.14 extracted from

  • Lin, L.L.; Chen, M.H.; Chien, H.C.; Kan, S.C.; Chen, C.C.; Hu, H.Y.; Hsu, W.H.
    Characterization of a bifunctional aminoacylase/carboxypeptidase from radioresistant bacterium Deinococcus radiodurans R1 (2007), J. Biotechnol., 128, 322-334.
    View publication on PubMed
Search for more literature for 3.5.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Deinococcus radiodurans

Inhibitors

Inhibitors Comment Organism Structure
Nac-L-Met
-
 Deinococcus radiodurans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3
-
 N-CBZ-Gly-Ala
-
 Deinococcus radiodurans

Metals/Ions

Metals/Ions Comment Organism Structure
CoCl2 relative activity of ACY is 286.2% when dipicolinic acid is removed Deinococcus radiodurans
CoCl2 relative activity of ACY is 310% when EDTA is removed, 40.2% with EDTA Deinococcus radiodurans
CuCl2 relative activity of ACY is 22.9% when dipicolinic acid is removed Deinococcus radiodurans
CuCl2 relative activity of ACY is 26.4% when EDTA is removed Deinococcus radiodurans
MnCl2 relative activity of ACY is 125.3% when dipicolinic acid is removed Deinococcus radiodurans
MnCl2 relative activity of ACY is 203.5% when EDTA is removed Deinococcus radiodurans
NiCl2 relative activity of ACY is 180.5% when dipicolinic acid is removed Deinococcus radiodurans
NiCl2 relative activity of ACY is 200.1% when EDTA is removed Deinococcus radiodurans
ZnCl2 relative activity of ACY is 113.7% when EDTA is removed Deinococcus radiodurans
ZnCl2 relative activity of ACY is 90.8% when dipicolinic acid is removed Deinococcus radiodurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 bifunctional DR_ACY/CP is purified by SDS-PAGE Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Deinococcus radiodurans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
 N-acetyl-D-Phe 8 mM Deinococcus radiodurans
0.37
-
 N-CBZ-Ile-Pro 6 mM Deinococcus radiodurans
0.55
-
 for DR_ACY/CP Deinococcus radiodurans
0.55
-
 N-acetyl-L-Trp 8 mM Deinococcus radiodurans
1.16
-
 N-CBZ-Ala-Glu 6 mM Deinococcus radiodurans
1.74
-
 N-acetyl-L-homophenylalanine 8 mM Deinococcus radiodurans
2.49
-
 N-CBZ-L-Ser 6 mM Deinococcus radiodurans
2.52
-
 N-acetyl-L-Leu 8 mM Deinococcus radiodurans
2.95
-
 N-CBZ-L-Phe 6 mM Deinococcus radiodurans
5.27
-
 N-CBZ-Gly-Gly-Phe 6 mM Deinococcus radiodurans
5.32
-
 N-CBZ-L-Glu 6 mM Deinococcus radiodurans
5.58
-
 N-CBZ-L-His 6 mM Deinococcus radiodurans
5.73
-
 for ACY Deinococcus radiodurans
5.73
-
 N-acetyl-L-Met 8 mM Deinococcus radiodurans
6.84
-
 N-chloroacetyl-L-Phe 8 mM Deinococcus radiodurans
8.07
-
 N-CBZ-Gly-Leu 6 mM Deinococcus radiodurans
8.4
-
 N-acetyl-L-Phe 8 mM Deinococcus radiodurans
8.54
-
 N-CBZ-Ala-Met 6 mM Deinococcus radiodurans
10.7
-
 N-CBZ-Gly-Tyr 6 mM Deinococcus radiodurans
11.26
-
 N-acetyl-L-His 8 mM Deinococcus radiodurans
15.1
-
 N-CBZ-Gly-Ala 6 mM Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-CBZ-Gly-Ala + H2O
-
 Deinococcus radiodurans ?
-
 ?
N-Cbz-Gly-L-Ala + H2O
-
 Deinococcus radiodurans ?
-
 ?

Synonyms

Synonyms Comment Organism
DR_ACY
-
 Deinococcus radiodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Deinococcus radiodurans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60
-
 Deinococcus radiodurans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 N-Cbz-Gly-L-Ala
-
 Deinococcus radiodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Deinococcus radiodurans

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Deinococcus radiodurans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
247
-
 Nac-L-Met
-
 Deinococcus radiodurans