General Stability | Organism |
---|---|
Co2+ activates and stabilizes | Streptomyces mobaraensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 24% inhibition | Streptomyces mobaraensis | |
EDTA | 13% inhibition | Streptomyces mobaraensis | |
additional information | no effects by GSH, L-Cys, (NH4)6Mo7O24, PMSF, PCMB, DTT, MgSO4, AgNO3, CaCl2, FeSO4, ZnCl2, and CuSO4 | Streptomyces mobaraensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates and stabilizes, 148% activity at 0.1 mM, tightly bound to the enzyme | Streptomyces mobaraensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer | Streptomyces mobaraensis |
61000 | - |
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer | Streptomyces mobaraensis |
75000 | - |
gel filtration | Streptomyces mobaraensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces mobaraensis | - |
i.e. Streptomyces luteoreticuli, strains NBRC/IFO 13819 and 13422 | - |
Purification (Comment) | Organism |
---|---|
native enzyme 1300fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, hydroxyapatite chromatography, and a second step of dialysis | Streptomyces mobaraensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22 | - |
purified enzyme, substrate N-lauroyl-L-tryptophan | Streptomyces mobaraensis |
25 | - |
purified enzyme, substrate N-lauroyl-L-phenylalanine | Streptomyces mobaraensis |
70 | - |
purified enzyme, substrate N-lauroyl-L-glutaminyl-L-lysine | Streptomyces mobaraensis |
87 | - |
purified enzyme, substrate N-lauroyl-L-valine | Streptomyces mobaraensis |
90 | - |
purified enzyme, substrate N-lauroyl-L-glutamyl-glycine | Streptomyces mobaraensis |
90 | - |
purified enzyme, substrate N-lauroyl-L-isoleucine | Streptomyces mobaraensis |
110 | - |
purified enzyme, substrate N-lauroyl-L-glutaminyl-glycine | Streptomyces mobaraensis |
118 | - |
purified enzyme, substrate N-lauroyl-L-histidine | Streptomyces mobaraensis |
129 | - |
purified enzyme, substrate N-lauroyl-L-leucine | Streptomyces mobaraensis |
138 | - |
purified enzyme, substrate N-lauroyl-L-aspartic acid | Streptomyces mobaraensis |
145 | - |
purified enzyme, substrate N-lauroyl-L-tyrosine | Streptomyces mobaraensis |
177 | - |
purified enzyme, substrate N-lauroyl-L-methionine | Streptomyces mobaraensis |
194 | - |
purified enzyme, substrate N-lauroyl-L-arginine | Streptomyces mobaraensis |
196 | - |
purified enzyme, substrate N-lauroyl-L-glutamic acid | Streptomyces mobaraensis |
210 | - |
purified enzyme, substrate capsaicin | Streptomyces mobaraensis |
221 | - |
purified enzyme, substrate N-lauroyl-L-glutamine | Streptomyces mobaraensis |
222 | - |
purified enzyme, substrate N-lauroyl-glycine | Streptomyces mobaraensis |
227 | - |
purified enzyme, substrate N-lauroyl-L-asparagine | Streptomyces mobaraensis |
230 | - |
purified enzyme, substrate N-lauroyl-L-threonine | Streptomyces mobaraensis |
236 | - |
purified enzyme, substrate N-lauroyl-L-alanine | Streptomyces mobaraensis |
244 | - |
purified enzyme, substrate N-lauroyl-L-lysine | Streptomyces mobaraensis |
295 | - |
purified enzyme, substrate N-lauroyl-L-serine | Streptomyces mobaraensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-methyl-N-vanillyl-6-nonenamide + H2O | i.e. capsaicin | Streptomyces mobaraensis | ? | - |
? | |
additional information | substrate synthesis in an aqueous/n-hexane biphasic system | Streptomyces mobaraensis | ? | - |
? | |
N-lauroyl-glycine + H2O | - |
Streptomyces mobaraensis | laurate + glycine | - |
? | |
N-lauroyl-L-alanine + H2O | - |
Streptomyces mobaraensis | laurate + L-alanine | - |
? | |
N-lauroyl-L-arginine + H2O | - |
Streptomyces mobaraensis | laurate + L-arginine | - |
? | |
N-lauroyl-L-asparagine + H2O | - |
Streptomyces mobaraensis | laurate + L-asparagine | - |
? | |
N-lauroyl-L-aspartate + H2O | - |
Streptomyces mobaraensis | laurate + L-aspartate | - |
? | |
N-lauroyl-L-glutamate + H2O | - |
Streptomyces mobaraensis | laurate + L-glutamate | - |
? | |
N-lauroyl-L-glutamine + H2O | - |
Streptomyces mobaraensis | laurate + L-glutamine | - |
? | |
N-lauroyl-L-glutaminyl-glycine + H2O | - |
Streptomyces mobaraensis | laurate + L-glutaminyl-glycine | - |
? | |
N-lauroyl-L-glutaminyl-L-lysine + H2O | - |
Streptomyces mobaraensis | laurate + L-glutaminyl-L-lysine | - |
? | |
N-lauroyl-L-glutamyl-glycine + H2O | - |
Streptomyces mobaraensis | laurate + L-glutamyl-glycine | - |
? | |
N-lauroyl-L-histidine + H2O | - |
Streptomyces mobaraensis | laurate + L-histidine | - |
? | |
N-lauroyl-L-isoleucine + H2O | - |
Streptomyces mobaraensis | laurate + L-isoleucine | - |
? | |
N-lauroyl-L-leucine + H2O | - |
Streptomyces mobaraensis | laurate + L-leucine | - |
? | |
N-lauroyl-L-methionine + H2O | - |
Streptomyces mobaraensis | laurate + L-methionine | - |
? | |
N-lauroyl-L-phenylalanine + H2O | - |
Streptomyces mobaraensis | laurate + L-phenylalanine | - |
? | |
N-lauroyl-L-serine + H2O | best substrate | Streptomyces mobaraensis | laurate + L-serine | - |
? | |
N-lauroyl-L-threonine + H2O | - |
Streptomyces mobaraensis | laurate + L-threonine | - |
? | |
N-lauroyl-L-tryptophan + H2O | - |
Streptomyces mobaraensis | laurate + L-tryptophan | - |
? | |
N-lauroyl-L-tyrosine + H2O | - |
Streptomyces mobaraensis | laurate + L-tyrosine | - |
? | |
N-lauroyl-L-valine + H2O | - |
Streptomyces mobaraensis | laurate + L-valine | - |
? | |
Nalpha-lauroyl-L-lysine + H2O | - |
Streptomyces mobaraensis | laurate + L-lysine | - |
? | |
Nepsilon-lauroyl-L-lysine + H2O | - |
Streptomyces mobaraensis | laurate + L-lysine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer | Streptomyces mobaraensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Streptomyces mobaraensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
1 h, pH 7.8, stable, above 55°C the enzyme is stable only in presence of Co2+ | Streptomyces mobaraensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces mobaraensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 50 mm Tris, 0.5 mM Co2+, 37°C, stable | Streptomyces mobaraensis |