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Literature summary for 3.5.1.14 extracted from

  • Henseling, J.; Roehm, K.H.
    Unusual solvent isotope effects on the aminoacylase-catalyzed hydrolysis of acetylamino acids (1987), FEBS Lett., 219, 27-30.
    View publication on PubMed
Search for more literature for 3.5.1.14

Application

Application Comment Organism
analysis enzyme widely used as reagent to resolve amino acid racemates Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.99
-
 N-acetyl-L-methionine
-
 Sus scrofa
1.62
-
 N-acetyl-L-methionine ordinary water, 50 mM MOPS 50 mM bicine buffer Sus scrofa
1.87
-
 N-acetyl-L-methionine hydrolysis in D2O, 50 mM MOPS 50 mM bicine buffer Sus scrofa
5.5
-
 N-acetyl-L-phenylalanine ordinary water, MOPS/NaOH buffer Sus scrofa
5.6
-
 N-acetyl-L-phenylalanine hydrolysis in D2O, MOPS/NaOH buffer, distinctly inverse deuterium isotope effect Sus scrofa
7
-
 N-acetyl-L-glutamine hydrolysis in D2O, MOPS/NaOH buffer Sus scrofa
10.2
-
 N-acetyl-L-glutamine MOPS/NaOH buffer Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 pig
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
N-acetyl-L-glutamine + H2O
-
 Sus scrofa L-glutamine + acetate
-
 ?
N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
N-acetyl-L-phenylalanine + H2O
-
 Sus scrofa L-phenylalanine + acetate
-
 ?

Synonyms

Synonyms Comment Organism
hog kidney aminoacylase I
-
 Sus scrofa