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Literature summary for 3.5.1.14 extracted from
- Aminoacylase from Aspergillus oryzae. Comparison with the pig kidney enzyme (1980), Z. Naturforsch. C, 35, 544-550.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Sus scrofa |  |
| Cd2+ | - |
Aspergillus oryzae | |
| Cu2+ | - |
Aspergillus oryzae | |
| diethyldicarbonate | - |
Aspergillus oryzae | |
| diethyldicarbonate | - |
Sus scrofa | |
| Mn2+ | - |
Aspergillus oryzae | |
| N-alpha-p-Tosyl-L-lysine | - |
Sus scrofa | |
| Ni2+ | - |
Aspergillus oryzae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | enzyme strongly activated by Co2+ ions | Sus scrofa | |
| Co2+ | enzyme strongly activated by Co2+ ions | Aspergillus oryzae | |
| Zn2+ | enzyme contains Zn as prosthetic metal | Sus scrofa | |
| Zn2+ | enzyme contains Zn as prosthetic metal | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36600 | - |
2 * 36600, SDS-PAGE | Aspergillus oryzae |
| 73200 | - |
- |
Aspergillus oryzae |
| 85500 | - |
- |
Sus scrofa |
| 135000 | - |
gel filtration | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-L-methionine | Sus scrofa | - |
L-methionine + acetate | - |
? | |
| N-acetyl-L-methionine | Aspergillus oryzae | - |
L-methionine + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.03 M phosphate buffer, 0.4 M NaCl, pH 7.3 may be stored for several months with negligible loss of activity | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-DL-methionine + H2O | - |
Sus scrofa | DL-methionine + acetate | - |
? | |
| N-acetyl-DL-methionine + H2O | - |
Aspergillus oryzae | DL-methionine + acetate | - |
? | |
| N-acetyl-L-methionine | - |
Sus scrofa | L-methionine + acetate | - |
? | |
| N-acetyl-L-methionine | - |
Aspergillus oryzae | L-methionine + acetate | - |
? | |
| N-chloroacetylglutamine-p-aminobenzoate + H2O | - |
Aspergillus oryzae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36600, SDS-PAGE | Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
denaturation at 60°C | Sus scrofa |
| 60 | - |
denaturation at 60°C | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Sus scrofa |
| 8.5 | - |
- |
Sus scrofa |
| 8.5 | - |
N-chloroacetyl-alanine as substrate, Zn2+ enzyme | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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