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Literature summary for 3.5.1.14 extracted from

  • Matsumoto, J.; Nagai, S.
    Amidohydrolases for N-short and long chain fatty acyl-L-amino acids from Mycobacteria (1972), J. Biochem., 72, 269-279.
    View publication on PubMed
Search for more literature for 3.5.1.14

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol with palmitoylaspartate as substrate Mycobacterium sp.
dithiothreitol slight activation of short acyl aminoacylase at 0.2 mM Mycobacterium sp.
mercaptoethanol
-
 Mycobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ inhibitory at concentration 1 mM Mycobacterium sp.
Cu2+
-
 Mycobacterium sp.
Hg2+
-
 Mycobacterium sp.
iodoacetic acid
-
 Mycobacterium sp.
Mn2+ inhibitory at concentration 1 mM Mycobacterium sp.
p-chloromercuribenzoate
-
 Mycobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 palmitoylaspartate
-
 Mycobacterium sp.
2.5
-
 N-acetyl-L-valine
-
 Mycobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Mycobacterium sp.
Co2+ enzyme strongly activated by Co2+ ions Mycobacterium sp.
Co2+ inhibitory at concentration 1 mM Mycobacterium sp.
Mg2+
-
 Mycobacterium sp.
Mn2+ inhibitory at concentration 1 mM Mycobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration, long acyl aminoacylase, hydrolysis of N-long chain fatty acyl amino acids Mycobacterium sp.
88000
-
 gel filtration, short acyl aminoacylase Mycobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-L-methionine Mycobacterium sp.
-
 L-methionine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium sp.
-
 avium, smegmatis, phlei
-

Purification (Commentary)

Purification (Comment) Organism
both short acyl aminoacylase and long acyl aminoacylase Mycobacterium sp.

Storage Stability

Storage Stability Organism
-20°C, 0.2 mM DTT buffer, pH 6.8, retains its acivity for 6 months Mycobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-DL-aspartate + H2O
-
 Mycobacterium sp. acetate + DL-aspartate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Mycobacterium sp. DL-methionine + acetate
-
 ?
N-acetyl-L-glutamine + H2O
-
 Mycobacterium sp. L-glutamine + acetate
-
 ?
N-acetyl-L-leucine + H2O
-
 Mycobacterium sp. acetate + L-leucine
-
 ?
N-acetyl-L-methionine
-
 Mycobacterium sp. L-methionine + acetate
-
 ?
N-acetyl-L-valine + H2O
-
 Mycobacterium sp. L-valine + acetate
-
 ?
palmitoyl-L-aspartate + H2O
-
 Mycobacterium sp. palmitate + L-aspartate
-
 ?
palmitoyl-L-phenylalanine + H2O
-
 Mycobacterium sp. palmitate + L-phenylalanine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
 with palmitoylaspartate as substrate Mycobacterium sp.