Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Corynebacterium striatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Corynebacterium striatum | |
EDTA | - |
Corynebacterium striatum | |
Mn2+ | partial loss of activity is observed with the combination Mn2+ | Corynebacterium striatum | |
additional information | not inhibited by phenylmethylsulfonyl fluoride and Pefablock (4-(2-aminoethyl)-benzenesulfonyl fluoride) or with pepstatin | Corynebacterium striatum | |
o-phenanthroline | - |
Corynebacterium striatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
benzyloxycarbonyl-L-glutamine | at pH 7.0 and 36°C | Corynebacterium striatum | |
0.06 | - |
Nalpha-lauroyl-L-glutamine | at pH 7.0 and 36°C | Corynebacterium striatum | |
0.08 | - |
Nalpha-decanoyl-L-glutamine | at pH 7.0 and 36°C | Corynebacterium striatum | |
0.2 | - |
(E)-3-methyl-2-hexenoyl-L-glutamine | at pH 7.0 and 36°C | Corynebacterium striatum | |
0.74 | - |
3-methyl-3-hydroxyhexanoyl-L-glutamine | at pH 7.0 and 36°C | Corynebacterium striatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent on | Corynebacterium striatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an Nalpha-acyl-L-glutamine + H2O | Corynebacterium striatum | the enzyme is highly specific for the glutamine residue but has a low specificity for the acyl part of the substrate | L-glutamine + a carboxylate | - |
? | |
an Nalpha-acyl-L-glutamine + H2O | Corynebacterium striatum AX20 | the enzyme is highly specific for the glutamine residue but has a low specificity for the acyl part of the substrate | L-glutamine + a carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium striatum | - |
- |
- |
Corynebacterium striatum AX20 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose CL-6B column chromatography, phenyl Sepharose column chromatography, and Mono Q column chromatography | Corynebacterium striatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-3-methyl-2-hexenoyl-L-glutamine + H2O | - |
Corynebacterium striatum | L-glutamine + (E)-3-methyl-2-hexenoic acid | - |
? | |
(E)-3-methyl-2-hexenoyl-L-glutamine + H2O | - |
Corynebacterium striatum AX20 | L-glutamine + (E)-3-methyl-2-hexenoic acid | - |
? | |
3-methyl-3-hydroxyhexanoyl-L-glutamine + H2O | - |
Corynebacterium striatum | L-glutamine + 3-methyl-3-hydroxyhexanoic acid | - |
? | |
3-methyl-3-hydroxyhexanoyl-L-glutamine + H2O | - |
Corynebacterium striatum AX20 | L-glutamine + 3-methyl-3-hydroxyhexanoic acid | - |
? | |
an Nalpha-acyl-L-glutamine + H2O | the enzyme is highly specific for the glutamine residue but has a low specificity for the acyl part of the substrate | Corynebacterium striatum | L-glutamine + a carboxylate | - |
? | |
an Nalpha-acyl-L-glutamine + H2O | the enzyme is highly specific for the glutamine residue but has a low specificity for the acyl part of the substrate | Corynebacterium striatum AX20 | L-glutamine + a carboxylate | - |
? | |
benzyloxycarbonyl-L-glutamine + H2O | lowest activity | Corynebacterium striatum | L-glutamine + benzyloxycarbonate | - |
? | |
benzyloxycarbonyl-L-glutamine + H2O | lowest activity | Corynebacterium striatum AX20 | L-glutamine + benzyloxycarbonate | - |
? | |
additional information | the enzyme shows very weak activity for Z-Ala. No activity for Z-L-asparagine, Z-L-aspartate, N-lauroyl-L-aspartate, Z-L-glutamate nor is there any cleavage of Z-D-glutamine, the methyl ester of Z-L-glutamine or Z-Gln-Gly | Corynebacterium striatum | ? | - |
? | |
additional information | the enzyme shows very weak activity for Z-Ala. No activity for Z-L-asparagine, Z-L-aspartate, N-lauroyl-L-aspartate, Z-L-glutamate nor is there any cleavage of Z-D-glutamine, the methyl ester of Z-L-glutamine or Z-Gln-Gly | Corynebacterium striatum AX20 | ? | - |
? | |
Nalpha-decanoyl-L-glutamine + H2O | highest activity | Corynebacterium striatum | L-glutamine + decanoic acid | - |
? | |
Nalpha-lauroyl-L-glutamine + H2O | - |
Corynebacterium striatum | L-glutamine + lauric acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Corynebacterium striatum |
? | x * 43365, nano-ESI-MS analysis | Corynebacterium striatum |
Synonyms | Comment | Organism |
---|---|---|
acylglutamine-aminoacylase | - |
Corynebacterium striatum |
AgaA | - |
Corynebacterium striatum |
N-acyl-Gln aminoacylase | - |
Corynebacterium striatum |