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Literature summary for 3.5.1.133 extracted from

  • Natsch, A.; Gfeller, H.; Gygax, P.; Schmid, J.; Acuna, G.
    A specific bacterial aminoacylase cleaves odorant precursors secreted in the human axilla (2003), J. Biol. Chem., 278, 5718-5727 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Corynebacterium striatum

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
Corynebacterium striatum
EDTA
-
Corynebacterium striatum
Mn2+ partial loss of activity is observed with the combination Mn2+ Corynebacterium striatum
additional information not inhibited by phenylmethylsulfonyl fluoride and Pefablock (4-(2-aminoethyl)-benzenesulfonyl fluoride) or with pepstatin Corynebacterium striatum
o-phenanthroline
-
Corynebacterium striatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
benzyloxycarbonyl-L-glutamine at pH 7.0 and 36°C Corynebacterium striatum
0.06
-
Nalpha-lauroyl-L-glutamine at pH 7.0 and 36°C Corynebacterium striatum
0.08
-
Nalpha-decanoyl-L-glutamine at pH 7.0 and 36°C Corynebacterium striatum
0.2
-
(E)-3-methyl-2-hexenoyl-L-glutamine at pH 7.0 and 36°C Corynebacterium striatum
0.74
-
3-methyl-3-hydroxyhexanoyl-L-glutamine at pH 7.0 and 36°C Corynebacterium striatum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Corynebacterium striatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an Nalpha-acyl-L-glutamine + H2O Corynebacterium striatum the enzyme is highly specific for the glutamine residue but has a low specificity for the acyl part of the substrate L-glutamine + a carboxylate
-
?
an Nalpha-acyl-L-glutamine + H2O Corynebacterium striatum AX20 the enzyme is highly specific for the glutamine residue but has a low specificity for the acyl part of the substrate L-glutamine + a carboxylate
-
?

Organism

Organism UniProt Comment Textmining
Corynebacterium striatum
-
-
-
Corynebacterium striatum AX20
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose CL-6B column chromatography, phenyl Sepharose column chromatography, and Mono Q column chromatography Corynebacterium striatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-3-methyl-2-hexenoyl-L-glutamine + H2O
-
Corynebacterium striatum L-glutamine + (E)-3-methyl-2-hexenoic acid
-
?
(E)-3-methyl-2-hexenoyl-L-glutamine + H2O
-
Corynebacterium striatum AX20 L-glutamine + (E)-3-methyl-2-hexenoic acid
-
?
3-methyl-3-hydroxyhexanoyl-L-glutamine + H2O
-
Corynebacterium striatum L-glutamine + 3-methyl-3-hydroxyhexanoic acid
-
?
3-methyl-3-hydroxyhexanoyl-L-glutamine + H2O
-
Corynebacterium striatum AX20 L-glutamine + 3-methyl-3-hydroxyhexanoic acid
-
?
an Nalpha-acyl-L-glutamine + H2O the enzyme is highly specific for the glutamine residue but has a low specificity for the acyl part of the substrate Corynebacterium striatum L-glutamine + a carboxylate
-
?
an Nalpha-acyl-L-glutamine + H2O the enzyme is highly specific for the glutamine residue but has a low specificity for the acyl part of the substrate Corynebacterium striatum AX20 L-glutamine + a carboxylate
-
?
benzyloxycarbonyl-L-glutamine + H2O lowest activity Corynebacterium striatum L-glutamine + benzyloxycarbonate
-
?
benzyloxycarbonyl-L-glutamine + H2O lowest activity Corynebacterium striatum AX20 L-glutamine + benzyloxycarbonate
-
?
additional information the enzyme shows very weak activity for Z-Ala. No activity for Z-L-asparagine, Z-L-aspartate, N-lauroyl-L-aspartate, Z-L-glutamate nor is there any cleavage of Z-D-glutamine, the methyl ester of Z-L-glutamine or Z-Gln-Gly Corynebacterium striatum ?
-
?
additional information the enzyme shows very weak activity for Z-Ala. No activity for Z-L-asparagine, Z-L-aspartate, N-lauroyl-L-aspartate, Z-L-glutamate nor is there any cleavage of Z-D-glutamine, the methyl ester of Z-L-glutamine or Z-Gln-Gly Corynebacterium striatum AX20 ?
-
?
Nalpha-decanoyl-L-glutamine + H2O highest activity Corynebacterium striatum L-glutamine + decanoic acid
-
?
Nalpha-lauroyl-L-glutamine + H2O
-
Corynebacterium striatum L-glutamine + lauric acid
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Corynebacterium striatum
? x * 43365, nano-ESI-MS analysis Corynebacterium striatum

Synonyms

Synonyms Comment Organism
acylglutamine-aminoacylase
-
Corynebacterium striatum
AgaA
-
Corynebacterium striatum
N-acyl-Gln aminoacylase
-
Corynebacterium striatum