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Literature summary for 3.5.1.13 extracted from

  • Hoagland, R.E.
    Hydrolysis of 3',4'-dichloropropionanilide by an aryl acylamidase from Taraxacum officinale (1975), Phytochemistry, 14, 383-386.
No PubMed abstract available
Search for more literature for 3.5.1.13

Inhibitors

Inhibitors Comment Organism Structure
catechol 0.5 mM, 73% inhibition Taraxacum officinale
CoCl2 1.0 mM, 16% inhibition Taraxacum officinale
Cu2+ 0.5 mM CuCl2, 86% inhibition Taraxacum officinale
FeCl3 1.0 mM, 6% inhibition Taraxacum officinale
HgCl2 0.5 mM, 89% inhibition Taraxacum officinale
NEM 1.0 mM, 23% inhibition Taraxacum officinale
o-Iodosobenzoate 0.25 mM, 27% inhibition Taraxacum officinale
p-benzoquinone 0.25 mM, 50% inhibition Taraxacum officinale
PCMB 0.25 mM, 61% inhibition Taraxacum officinale

Organism

Organism UniProt Comment Textmining
Taraxacum officinale
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Taraxacum officinale

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Taraxacum officinale
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dichloropropionanilide + H2O 14% of the activity with 3',4'-dichloropropionanilide Taraxacum officinale 2,3-dichloroaniline + propionate
-
 ?
2',4'-dichloropropionanilide + H2O 49% of the activity with 3',4'-dichloropropionanilide Taraxacum officinale 2,4-dichloroaniline + propionate
-
 ?
2'-chloropropionanilide + H2O 37% of the activity with 3',4'-dichloropropionanilide Taraxacum officinale 2-chloroaniline + propionate
-
 ?
3',4'-dichloro-2-methacrylanilide + H2O 8% of the activity with 3',4'-dichloropropionanilide Taraxacum officinale 3,4-dichloroaniline + 2-methyl-2-propenoate
-
 ?
3',4'-dichloroacetanilide + H2O 49% of the activity with 3',4'-dichloropropionanilide Taraxacum officinale 3,4-dichloroaniline + acetate
-
 ?
3',4'-dichlorobutyranilide + H2O 37% of the activity with 3',4'-dichloropropionanilide Taraxacum officinale 3,4-dichloroaniline + butyrate
-
 ?
3',4'-dichloropropionanilide + H2O
-
 Taraxacum officinale 3,4-dichloroaniline + propionate
-
 ?
3',4'-dichlorovaleranilide + H2O 12% of the activity with 3',4'-dichloropropionanilide Taraxacum officinale 3,4-dichloroaniline + pentanoate
-
 ?
3'-chloropropionanilide + H2O 51% of the activity with 3',4'-dichloropropionanilide Taraxacum officinale 3-chloroaniline + propionate
-
 ?
4'-chloropropionanilide + H2O 54% of the activity with 3',4'-dichloropropionanilide Taraxacum officinale 4-chloroaniline + propionate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Taraxacum officinale

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 5 min, enzyme retains high activity Taraxacum officinale
46 48 5 min, 50% loss of activity Taraxacum officinale
68
-
 5 min, complete inactivation Taraxacum officinale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.8
-
 Taraxacum officinale

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.8 pH 6.2: 70% of maximal activity, pH 8.8: 84% of maximal activity Taraxacum officinale

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.25
-
 p-benzoquinone
-
 Taraxacum officinale