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Literature summary for 3.5.1.12 extracted from

  • Thoma, R.W.; Peterson, W.H.
    The enzymatic degradation of soluble bound biotin (1954), J. Biol. Chem., 210, 569-579.
    View publication on PubMed
Search for more literature for 3.5.1.12

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activator might be need to give full release of biotin, erratic effect Sus scrofa
Zn2+ activator might be need to give full release of biotin, erratic effect Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biotin amide + H2O Gallus sp.
-
 ?
-
 ?
biotin amide + H2O Sus scrofa soluble bound biotin ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O Gallus sp.
-
 ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O Sus scrofa biocytin, soluble bound biotin ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol no loss of enzyme occurs in 10% at 0°C, at 50% all activity lost Sus scrofa

Organism

Organism UniProt Comment Textmining
Gallus sp.
-
 chicken
-
no activity in Lactobacillus arabinosus
-
-
-
Sus scrofa
-
 pig
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-
liver
-
 Sus scrofa
-
pancreas
-
 Gallus sp.
-

Storage Stability

Storage Stability Organism
15°C enzyme solution with toluene, for a week or more without appreciable loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biotin amide + H2O
-
 Gallus sp. ?
-
 ?
biotin amide + H2O soluble bound biotin Sus scrofa ?
-
 ?
biotin methyl ester + H2O
-
 Sus scrofa ?
-
 ?
biotin methyl ester + H2O
-
 Gallus sp. ?
-
 ?
biotinyl-L-aspartate + H2O
-
 Sus scrofa biotin + L-aspartate
-
 ?
biotinyl-L-aspartate + H2O
-
 Gallus sp. biotin + L-aspartate
-
 ?
epsilon-N-biotinyl-L-lysine + H2O
-
 Gallus sp. biotin + L-lysine
-
 ir
epsilon-N-biotinyl-L-lysine + H2O biocytin, soluble bound biotin Sus scrofa biotin + L-lysine
-
 ir
epsilon-N-biotinyl-L-lysine + H2O
-
 Gallus sp. ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O biocytin, soluble bound biotin Sus scrofa ?
-
 ?
N-biotinyl-beta-alanine + H2O
-
 Sus scrofa biotin + beta-alanine
-
 ?
N-biotinyl-beta-alanine + H2O
-
 Gallus sp. biotin + beta-alanine
-
 ?
p-(N-biotinylamino)benzoic acid + H2O
-
 Sus scrofa biotin + p-aminobenzoic acid
-
 ir
p-(N-biotinylamino)benzoic acid + H2O
-
 Gallus sp. biotin + p-aminobenzoic acid
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5
-
 Sus scrofa