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Literature summary for 3.5.1.11 extracted from
- Penicillin acylase immobilization depending on macromolecular crowding and catalysis in aqueous-organic medium (2009), Bioprocess Biosyst. Eng., 32, 765-772.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | penicillin acylase is one of the key pharmaceutical enzymes in the production of polysynthetic beta-lactam antibiotics | Priestia megaterium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of the enzyme on mesocellular silica foams from 0.1 M phosphate buffer, pH 7.0, the immobilized enzyme activity depends on molecular crowding and applied to catalysis in non-aqueous medium, addition of dextran, method optimization, using different enzyme preparations, organic solvents and mesocellular silica foams-dextran mixtures, overview | Priestia megaterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | Priestia megaterium | - |
phenylacetic acid + 6-aminopenicillanate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | 1000 U/ml, 10 mg/ml | Priestia megaterium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 182 | - |
immobilized enzyme, pH 8.0, 37°C | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cephalosporin G + H2O | - |
Priestia megaterium | phenylacetic acid + 7-aminodeacetoxycephalosporanate | - |
? | |
| penicillin G + H2O | - |
Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | - |
? | |
| penicillin G + H2O | - |
Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | detection by reaction with p-dimethyl-aminobenzaldehyde | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| penicillin acylase | - |
Priestia megaterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Priestia megaterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Priestia megaterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | The enzyme catalyzes the hydrolysis of the amidic bonds of penicillin G and cephalosporin G to produce key components in the synthesis of beta-lactam antibiotics | Priestia megaterium |
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