Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Priestia megaterium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20500 | - |
1 * 20500 + 1 * 69000, SDS-PAGE | Escherichia coli |
26900 | - |
1 * 26900 + 1 * 59100, SDS-PAGE | Priestia megaterium |
59100 | - |
1 * 26900 + 1 * 59100, SDS-PAGE | Priestia megaterium |
69000 | - |
1 * 20500 + 1 * 69000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | Escherichia coli | - |
phenylacetic acid + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | Priestia megaterium | - |
phenylacetic acid + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | Priestia megaterium ATCC 14945 | - |
phenylacetic acid + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | Escherichia coli ATCC 9637 | - |
phenylacetic acid + 6-aminopenicillanate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli ATCC 9637 | - |
- |
- |
Priestia megaterium | - |
- |
- |
Priestia megaterium ATCC 14945 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 12.7fold from fermentation broth by expanded bed adsorption method using a cationic resin, method comparisons show similar results for packed-bed and for expanded-bed modes, evaluation, overview | Priestia megaterium |
native enzyme 4.5-7.5fold from crude cell extract or homogenate by expanded bed adsorption method using a cationic resin, method comparisons show similar results for packed-bed and for expanded-bed modes, evaluation, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | - |
Escherichia coli | phenylacetic acid + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | - |
Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | - |
Escherichia coli | phenylacetic acid + 6-aminopenicillanate | product detection by p-dimethylamine benzaldehyde | ? | |
penicillin G + H2O | - |
Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | product detection by p-dimethylamine benzaldehyde | ? | |
penicillin G + H2O | - |
Priestia megaterium ATCC 14945 | phenylacetic acid + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | - |
Priestia megaterium ATCC 14945 | phenylacetic acid + 6-aminopenicillanate | product detection by p-dimethylamine benzaldehyde | ? | |
penicillin G + H2O | - |
Escherichia coli ATCC 9637 | phenylacetic acid + 6-aminopenicillanate | - |
? | |
penicillin G + H2O | - |
Escherichia coli ATCC 9637 | phenylacetic acid + 6-aminopenicillanate | product detection by p-dimethylamine benzaldehyde | ? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 20500 + 1 * 69000, SDS-PAGE | Escherichia coli |
heterodimer | 1 * 26900 + 1 * 59100, SDS-PAGE | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
penicillin G acylase | - |
Escherichia coli |
penicillin G acylase | - |
Priestia megaterium |
PGA | - |
Escherichia coli |
PGA | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
8 | - |
assay at | Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 4.8 | high inactivation at pH 4.0, but stable at pH 4.8 | Priestia megaterium |
4.5 | 4.8 | high inactivation at pH 4.5, but stable at pH 4.8 | Escherichia coli |