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Literature summary for 3.5.1.11 extracted from

  • Hormigo, D.; De La Mata, I.; Castillon, M.; Acebal, C.; Arroyo, M.
    Kinetic and microstructural characterization of immobilized penicillin acylase from Streptomyces lavendulae on Sepabeads EC-EP (2009), Biocatal. Biotransform., 27, 271-281.
No PubMed abstract available
Search for more literature for 3.5.1.11

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the recombinant enzyme by covalent linking to epoxy-activated beads in homogenous distribution to avoid enzyme aggregate formation, and usage as biocatalyst. The immobilized enzyme shows altered optimal reaction conditions and enlarged substrate specificity, as well as increased thermal stability compared to the soluble enzyme, it can be recycled 30times in consecutive batch reactions, overview Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
penicillin dihydroF + H2O Streptomyces lavendulae substrate of immobilized enzyme ?
-
 ?
penicillin F + H2O Streptomyces lavendulae substrate of immobilized enzyme ?
-
 ?
penicillin K + H2O Streptomyces lavendulae substrate of immobilized enzyme ?
-
 ?
penicillin V + H2O Streptomyces lavendulae substrate of soluble and immobilized enzyme 6-aminopenicillanic acid + phenoxyacetic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
penicillin dihydroF + H2O substrate of immobilized enzyme Streptomyces lavendulae ?
-
 ?
penicillin F + H2O substrate of immobilized enzyme Streptomyces lavendulae ?
-
 ?
penicillin K + H2O substrate of immobilized enzyme Streptomyces lavendulae ?
-
 ?
penicillin V + H2O substrate of soluble and immobilized enzyme Streptomyces lavendulae 6-aminopenicillanic acid + phenoxyacetic acid
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 soluble recombinant enzyme Streptomyces lavendulae
65
-
 immobilized recombinant enzyme Streptomyces lavendulae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 8.0, half-life of the soluble enzyme is 397 h, and for the immobilized enzyme 625 h Streptomyces lavendulae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 soluble recombinant enzyme Streptomyces lavendulae
8.5
-
 immobilized recombinant enzyme Streptomyces lavendulae

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 40°C, half-life of the soluble enzyme is 397 h, and for the immobilized enzyme 625 h Streptomyces lavendulae