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Literature summary for 3.5.1.11 extracted from

  • Polizzi, K.M.; Chaparro-Riggers, J.F.; Vazquez-Figueroa, E.; Bommarius, A.S.
    Structure-guided consensus approach to create a more thermostable penicillin G acylase (2006), Biotechnol. J., 1, 531-536.
    View publication on PubMed
Search for more literature for 3.5.1.11

Protein Variants

Protein Variants Comment Organism
A149Q activity is too low to measure thermal stability Escherichia coli
A305D mutation in beta-subunit. Half-life at 50°C is 1.6fold higher than wild-type value. Activity is about 60% of wild-type value Escherichia coli
A545K mutation in beta-subunit. Half-life at 50°C is about 80% of wild-type value. Activity is about 80% of wild-type value Escherichia coli
A80R mutation in alpha-subunit. Half-life at 50°C is 2.6fold higher than wild-type value. Activity is 1.6fold higher than wild-type value Escherichia coli
A84P mutation in beta-subunit. Half-life at 50°C is 1.3fold higher than wild-type value. Activity is identical to wild-type value Escherichia coli
E130T mutation in alpha-subunit. Half-life at 50°C is about 60% of wild-type value. Activity is about 1.2fold higher than wild-type value Escherichia coli
H26G activity is too low to measure thermal stability Escherichia coli
L100E mutation in beta-subunit. Half-life at 50°C is 1.2fold higher than wild-type value. Activity is about 50% of wild-type value Escherichia coli
M90R mutation in alpha-subunit. Half-life at 50°C is nearly identical to wild-type value. Activity is about 70% of wild-type value Escherichia coli
N348D mutation in beta-subunit. Half-life at 50°C is 1.5fold higher than wild-type value. Activity is nearly identical to wild-type value Escherichia coli
Q118E activity is too low to measure thermal stability Escherichia coli
S374T mutation in beta-subunit. Half-life at 50°C is about 90% of wild.type value. Activity about 50% of wild-type value Escherichia coli
S9E activity is too low to measure thermal stability Escherichia coli
T121D mutation in alpha-subunit. Half-life at 50°C is about 70% of wild-type value. Activity is about 40% of wild-type value Escherichia coli
T150N mutation in alpha-subunit. Half-life at 50°C is 1.5fold higher than wild-type value. Activity is about 50% of wild-type value Escherichia coli
T311P/Q312A mutation in beta-subunit. Half-life at 50°C is 2fold higher than wild-type value. Activity is about 90% of wild-type value Escherichia coli
V184K mutation in beta-subunit. Half-life at 50°C is about 60% of wild-type value. Activity is about 60% of wild-type value Escherichia coli
V359L mutation in beta-subunit. Half-life at 50°C is 1.4fold higher than wild-type value. Activity is about 60% of wild-type value Escherichia coli
V400L mutation in beta-subunit. Half-life at 50°C is 1.8fold higher than wild-type value. Activity is about 75% of wild-type value Escherichia coli
V97K activity is too low to measure thermal stability Escherichia coli
W25Y mutation in alpha-subunit. Half-life at 50°C is 2.7fold higher than wild-type value. Activity is 1.4fold higher than wild-type value Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P06875
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