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Literature summary for 3.5.1.11 extracted from
- Evaluation of penicillin acylase production by two strains of Bacillus megaterium (1993), Biol. Res., 26, 357-364.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Priestia megaterium | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | Priestia megaterium | catalysis of the reverse reaction at low pH or low water activity | phenylacetic acid + 6-aminopenicillanate | - |
r |  |
| penicillin V + H2O | Priestia megaterium | - |
phenoxyacetic acid + 6-aminopenicillanate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
strain ATCC 14945 and ATCC 14946, enzyme production is induced by phenylacetic acid, addition of glucose to the culture medium severely represses enzyme production in strain ATCC 14945 but not in ATCC 14946, higher activities using complex compared with defined medium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| patial | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | catalysis of the reverse reaction at low pH or low water activity | Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | - |
r | |
| penicillin V + H2O | - |
Priestia megaterium | phenoxyacetic acid + 6-aminopenicillanate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PA | - |
Priestia megaterium |
| Penicillin amidohydrolase | - |
Priestia megaterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
unstable at optimum temperature | Priestia megaterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | - |
Priestia megaterium |
| 37 | - |
75% of maximum activity | Priestia megaterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
fully stable at temperatures up to 40°C upon 1 h incubation at 8.5°C | Priestia megaterium |
| 50 | - |
60% of initial activity lost upon 1 h incubation at 8.5°C | Priestia megaterium |
| 57 | - |
unstable at the optimum temperature | Priestia megaterium |
| 60 | - |
almost complete inactivation upon 1 h incubation at 8.5°C | Priestia megaterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
broad optimum | Priestia megaterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | steep increase in activity up to pH 6.5, approximately 75% of activity at pH 10 | Priestia megaterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | almost fully stable upon incubation at 37°C | Priestia megaterium |
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