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Literature summary for 3.5.1.11 extracted from

  • Cheng, T.; Chen, M.; Zheng, H.; Wang, J.; Yang, S.; Jiang, W.
    Expression and purification of penicillin G acylase enzymes from four different micro-organisms, and a comparative evaluation of their synthesis/hydrolysis ratios for cephalexin (2006), Protein Expr. Purif., 46, 107-113.
    View publication on PubMed
Search for more literature for 3.5.1.11

Cloned(Commentary)

Cloned (Comment) Organism
-
 Alcaligenes faecalis
-
 Escherichia coli
-
 Providencia rettgeri
-
 Kluyvera cryocrescens

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Escherichia coli
-
-
-
Kluyvera cryocrescens
-
-
-
Providencia rettgeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged recombinant enzyme Alcaligenes faecalis
His-tagged recombinant enzyme Escherichia coli
His-tagged recombinant enzyme Providencia rettgeri
His-tagged recombinant enzyme Kluyvera cryocrescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1975
-
 30°C, pH 7.0 Providencia rettgeri
12840
-
 30°C, pH 7.0, high intrinsic synthseis/hydrolysis ratio Kluyvera cryocrescens
17990
-
 30°C, pH 7.0 Alcaligenes faecalis
22240
-
 30°C, pH 7.0 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid kinetically controlled synthesis Alcaligenes faecalis cephalexin + NH3
-
 r
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid kinetically controlled synthesis Escherichia coli cephalexin + NH3
-
 r
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid kinetically controlled synthesis Providencia rettgeri cephalexin + NH3
-
 r
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid kinetically controlled synthesis Kluyvera cryocrescens cephalexin + NH3
-
 r