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Literature summary for 3.5.1.11 extracted from
- Expression, purification, and characterization of His-tagged penicillin G acylase from Kluyvera citrophila in Escherichia coli (2004), Protein Expr. Purif., 38, 24-28.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0176 | - |
6-nitro-3-phenylacetamide benzoic acid | pH 7.5, 37°C | Kluyvera cryocrescens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 23000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE | Kluyvera cryocrescens |
| 62000 | - |
x * 23000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE | Kluyvera cryocrescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyvera cryocrescens | - |
recombinant enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tagged enzyme expressed in Escherichia coli | Kluyvera cryocrescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35.3 | - |
pH 7.5, 37°C | Kluyvera cryocrescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-nitro-3-phenylacetamide benzoic acid + H2O | - |
Kluyvera cryocrescens | 3-amino-6-nitrobenzoic acid + phenylacetic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE | Kluyvera cryocrescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Kluyvera cryocrescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
stable for at least 10 days | Kluyvera cryocrescens |
| 22 | - |
10 days, 76% residual activity | Kluyvera cryocrescens |
| 40 | - |
degradation above | Kluyvera cryocrescens |
| 60 | - |
30 min, complete loss of activity | Kluyvera cryocrescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Kluyvera cryocrescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
more than 40% of maximum activity | Kluyvera cryocrescens |
| 5 | 10 | plateau of activity within this range | Kluyvera cryocrescens |
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