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Literature summary for 3.5.1.11 extracted from

  • Guncheva, M.; Ivanov, I.; Galunsky, B.; Stambolieva, N.; Kaneti, J.
    Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 (2004), Eur. J. Biochem., 271, 2272-2279.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00001
-
 penicillin G 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
0.000012
-
 phenylacetyl-7-amido-4-methylcoumarin 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
0.000015
-
 6-nitro-3-[(phenylacetyl)amino]benzoic acid 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
0.0002
-
 phenylacetamide 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
0.0002
-
 phenylacetyl-2-naphthylamide 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
0.0003
-
 4-phenylacetamidobenzoic acid 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
0.0004
-
 phenylacetate 4-nitroanilide 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
0.0004
-
 3-phenylacetamidobenzoic acid 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
0.00051
-
 N-(5-nitro-2-pyridyl)-phenylacetamide 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
0.00063
-
 2-phenylacetamidobenzoic acid 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylacetamidobenzoic acid + H2O
-
 Escherichia coli phenylacetate + 2-aminobenzoic acid
-
 ?
3-phenylacetamidobenzoic acid + H2O
-
 Escherichia coli phenylacetate + 3-aminobenzoic acid
-
 ?
4-phenylacetamidobenzoic acid + H2O
-
 Escherichia coli phenylacetate + 4-aminobenzoic acid
-
 ?
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
-
 Escherichia coli 3-amino-6-nitrobenzoic acid + phenylacetic acid
-
 ?
N-(5-nitro-2-pyridyl)-phenylacetamide + H2O
-
 Escherichia coli phenylacetate + 5-nitropyridin-2-amine
-
 ?
penicillin G + H2O
-
 Escherichia coli 6-aminopenicillanic acid + phenylacetic acid
-
 ?
phenylacetamide + H2O
-
 Escherichia coli phenylacetate + NH3
-
 ?
phenylacetate 4-nitroanilide + H2O
-
 Escherichia coli phenylacetic acid + 4-nitrolaniline
-
 ?
phenylacetyl-2-naphthylamide + H2O
-
 Escherichia coli phenylacetate + 2-naphthylamine
-
 ?
phenylacetyl-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli phenylacetate + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 phenylacetyl-2-naphthylamide 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
2.3
-
 2-phenylacetamidobenzoic acid 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
2.5
-
 3-phenylacetamidobenzoic acid 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
3
-
 4-phenylacetamidobenzoic acid 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
3.2
-
 phenylacetyl-7-amido-4-methylcoumarin 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
7.8
-
 N-(5-nitro-2-pyridyl)-phenylacetamide 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
20
-
 6-nitro-3-[(phenylacetyl)amino]benzoic acid 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
20
-
 phenylacetate 4-nitroanilide 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
50
-
 penicillin G 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli
50
-
 phenylacetamide 25°C, pH 7.0, 10% dimethylsulfoxide Escherichia coli