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Literature summary for 3.5.1.11 extracted from

  • Terreni, M.; Ubiali, D.; Pagani, G.; Hernandez-Justiz, O.; Fernandez-Lafuente, R.; Guisan, J.M.
    Penicillin G acylase catalyzed acylation of 7-ACA in aqueous two-phase systems using kinetically and thermodynamically controlled strategies: improved enzymatic synthesis of 7-[(1-hydroxy-1-phenyl)-acetamido]-3-acetoxymethyl-D3-cephem-4-carboxylic acid (2005), Enzyme Microb. Technol., 36, 672-679.
No PubMed abstract available

Application

Application Comment Organism
synthesis improved synthesis of 3’-functionalized cephalosporins by enzyme in polyethylene glycol-ammonium sulfate aqueous two-phase systems. Best results are achieved using PEG 600, 80% in water, equilibrated with 4 M ammonium sulfate. Acylation product is completely partitioned in the PEG phase, whereas the substrates maintain a suitable concentration in the enzyme environment. Use for synthesis of cefamandole and cefonicid Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-aminocephalosporanic acid + (R)-mandelic acid
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Escherichia coli 7-[(1-hydroxy-1-phenyl)-acetamido]-3-acetoxymethyl-D3-cephem-4-carboxylic acid
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