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Literature summary for 3.5.1.11 extracted from

  • Gabor, E.M.; de Vries, E.J.; Janssen, D.B.
    A novel penicillin acylase from the environmental gene pool with improved synthetic properties (2005), Enzyme Microb. Technol., 36, 182-190.
No PubMed abstract available
Search for more literature for 3.5.1.11

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 6-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
0.012
-
 penicillin G pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
0.013
-
 penicillin G pH 7.0, 30°C Escherichia coli
0.015
-
 2-nitro-5-[(phenylacetyl)amino]-benzoic acid pH 7.0, 30°C Escherichia coli
0.027
-
 4-Hydroxyphenylacetamide pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
0.03
-
 phenylacetamide pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
0.114
-
 4-Hydroxyphenylacetamide pH 7.0, 30°C Escherichia coli
0.156
-
 phenylacetamide pH 7.0, 30°C Escherichia coli
0.284
-
 cefadroxil pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
0.399
-
 amoxicillin pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
0.575
-
 ampicillin pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
0.646
-
 cefadroxil pH 7.0, 30°C Escherichia coli
0.646
-
 D-2-nitro-5-[(phenylacetyl)amino]benzoic acid pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
1.07
-
 amoxicillin pH 7.0, 30°C Escherichia coli
1.3
-
 cephalexin pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
1.3
-
 D-2-nitro-5-[(phenylacetyl)amino]benzoic acid pH 7.0, 30°C Escherichia coli
1.5
-
 cephalexin pH 7.0, 30°C Escherichia coli
2.5
-
 ampicillin pH 7.0, 30°C Escherichia coli
9.1
-
 D-4-hydroxyphenylglycine amide pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
12
-
 D-phenylglycine amide pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
12.2
-
 D-4-hydroxyphenylglycine amide pH 7.0, 30°C Escherichia coli
30
-
 D-phenylglycine amide pH 7.0, 30°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
 x * 25500, alpha-subunit, + x * 61900, beta-subunit, SDS-PAGE uncultured Gammaproteobacteria bacterium
61900
-
 x * 25500, alpha-subunit, + x * 61900, beta-subunit, SDS-PAGE uncultured Gammaproteobacteria bacterium

Organism

Organism UniProt Comment Textmining
Escherichia coli P06875
-
-
uncultured Gammaproteobacteria bacterium Q6PWR5 TaxID 86473, growth on D-phenylglycine as sole source of nitrogen
-

Storage Stability

Storage Stability Organism
-20°C, enzyme may be defrosted several times without loss of activity uncultured Gammaproteobacteria bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylacetamide + 6-aminopenicillanic acid
-
 uncultured Gammaproteobacteria bacterium ?
-
 r
4-hydroxyphenylacetamide + 6-aminopenicillanic acid
-
 Escherichia coli ?
-
 r
6-aminopenicillanate + phenylacetic acid
-
 Escherichia coli penicillin G + H2O
-
 r
6-aminopenicillanate + phenylacetic acid synthesis of penicillin G by enzyme is significantly better than by Escherichia coli enzyme uncultured Gammaproteobacteria bacterium penicillin G + H2O
-
 r
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
-
 uncultured Gammaproteobacteria bacterium 3-amino-6-nitrobenzoic acid + phenylacetic acid
-
 r
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
-
 Escherichia coli 3-amino-6-nitrobenzoic acid + phenylacetic acid
-
 r
amoxillin + H2O
-
 uncultured Gammaproteobacteria bacterium D-4-hydroxyphenylglycine + 6-aminopenicillanic acid
-
 r
amoxillin + H2O
-
 Escherichia coli D-4-hydroxyphenylglycine + 6-aminopenicillanic acid
-
 r
ampicillin + H2O
-
 uncultured Gammaproteobacteria bacterium (R)-2-phenylglycine + 6-aminopenicillanic acid
-
 r
ampicillin + H2O
-
 Escherichia coli (R)-2-phenylglycine + 6-aminopenicillanic acid
-
 r
cefadroxil + H2O
-
 uncultured Gammaproteobacteria bacterium ?
-
 r
cefadroxil + H2O
-
 Escherichia coli ?
-
 r
cephalexin + H2O
-
 uncultured Gammaproteobacteria bacterium 7-aminodesacetoxycephalosporanic acid + D-phenylglycine
-
 r
cephalexin + H2O
-
 Escherichia coli 7-aminodesacetoxycephalosporanic acid + D-phenylglycine
-
 r
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
-
 uncultured Gammaproteobacteria bacterium phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
 r
D-2-nitro-5-[(phenylacetyl)amino]benzoic acid + H2O
-
 Escherichia coli phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
 r
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
-
 uncultured Gammaproteobacteria bacterium amoxicillin + NH3
-
 r
D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
-
 Escherichia coli amoxicillin + NH3
-
 r
D-phenylglycine amide + 6-aminopenicillanic acid
-
 uncultured Gammaproteobacteria bacterium ampicillin + NH3
-
 r
D-phenylglycine amide + 6-aminopenicillanic acid
-
 Escherichia coli ampicillin + NH3
-
 r
penicillin G + H2O
-
 uncultured Gammaproteobacteria bacterium 6-aminopenicillanate + phenylacetic acid
-
 r
penicillin G + H2O
-
 Escherichia coli 6-aminopenicillanate + phenylacetic acid
-
 r
phenylacetamide + 6-aminopenicillanic acid
-
 uncultured Gammaproteobacteria bacterium ?
-
 r
phenylacetamide + 6-aminopenicillanic acid
-
 Escherichia coli ?
-
 r

Subunits

Subunits Comment Organism
? x * 25500, alpha-subunit, + x * 61900, beta-subunit, SDS-PAGE uncultured Gammaproteobacteria bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 D-4-hydroxyphenylglycine amide pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma Escherichia coli
12
-
 D-2-nitro-5-[(phenylacetyl)amino]benzoic acid pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma uncultured Gammaproteobacteria bacterium
13
-
 cefadroxil pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
14
-
 D-2-nitro-5-[(phenylacetyl)amino]benzoic acid pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma Escherichia coli
15
-
 amoxicillin pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
16
-
 ampicillin pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
16
-
 D-4-hydroxyphenylglycine amide pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma uncultured Gammaproteobacteria bacterium
17
-
 amoxicillin pH 7.0, 30°C Escherichia coli
18
-
 2-nitro-5-[(phenylacetyl)amino]-benzoic acid pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma Escherichia coli
20
-
 cephalexin pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
23
-
 phenylacetamide pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma uncultured Gammaproteobacteria bacterium
24
-
 6-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma uncultured Gammaproteobacteria bacterium
25
-
 penicillin G pH 7.0, 30°C uncultured Gammaproteobacteria bacterium
25
-
 ampicillin pH 7.0, 30°C Escherichia coli
25
-
 D-phenylglycine amide pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma uncultured Gammaproteobacteria bacterium
29
-
 cephalexin pH 7.0, 30°C Escherichia coli
29
-
 4-Hydroxyphenylacetamide pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma uncultured Gammaproteobacteria bacterium
32
-
 cefadroxil pH 7.0, 30°C Escherichia coli
39
-
 penicillin G pH 7.0, 30°C Escherichia coli
46
-
 phenylacetamide pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma Escherichia coli
47
-
 4-Hydroxyphenylacetamide pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma Escherichia coli
57
-
 D-phenylglycine amide pH 7.0, 30°C, analysis of complex kinetic parameters alpha, beta, gamma Escherichia coli