Protein Variants | Comment | Organism |
---|---|---|
additional information | addition of a tag of three lysine residues alternating with three glycines to C-terminus of enzyme results in improvement of the immobilization efficiency on glyoxyl agarose and the catalytic protperties of immobilized enzyme, but impairs posttranslational steps of overexpressed protein maturation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
substrate (R)-mandelic acid methyl ester, mutant with three lysine residues alternating with three glycines at C-terminus, pH 8.5, 25°C | Escherichia coli |
12 | - |
substrate (R)-mandelic acid methyl ester, wild-type, pH 8.5, 25°C | Escherichia coli |
12 | - |
substrate 6-nitro-3-[(phenylacetyl)amino]benzoic acid, mutant with three lysine residues alternating with three glycines at C-terminus, pH 8.5, 25°C | Escherichia coli |
16 | - |
substrate 6-nitro-3-[(phenylacetyl)amino]benzoic acid, wild-type, pH 8.5, 25°C | Escherichia coli |
18 | - |
substrate penicillin G, mutant with three lysine residues alternating with three glycines at C-terminus, pH 8.5, 25°C | Escherichia coli |
33 | - |
substrate penicillin G, wild-type, pH 8.5, 25°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelic acid methyl ester + H2O | - |
Escherichia coli | ? | - |
? | |
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O | - |
Escherichia coli | 3-amino-6-nitrobenzoic acid + phenylacetic acid | - |
? | |
penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanate + phenylacetic acid | - |
? |