Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of chemically synthesized fragments of the pro-peptide to purified mature enzyme increases its specific activity up to 2.3fold | Alcaligenes faecalis |
Protein Variants | Comment | Organism |
---|---|---|
T206G | specific activity of the mutant enzyme is 60% higher compared to the wild-type enzyme, pI is 5.5 | Alcaligenes faecalis |
T206G/G213G | mutant enzyme with 1.9fold increased specific activity compared to completely processed wild-type enzyme, mutation stabilizes the precursor form, pI value is 5.6 | Alcaligenes faecalis |
T206G/S213G/T219G | mutant enzyme with 2.3fold increased specific activity compared to completely processed wild-type enzyme | Alcaligenes faecalis |
T206P | mutant undergoes normal proteolytic processing leading to a completely processed enzyme with pI 5.3 | Alcaligenes faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
benzylpenicillin | pH 7.5, 25°C, mutant enzyme T206G/S213G/T219G | Alcaligenes faecalis | |
0.007 | - |
benzylpenicillin | pH 7.5, 25°C, mutant enzyme T206G//S213G | Alcaligenes faecalis | |
0.008 | - |
benzylpenicillin | pH 7.5, 25°C, mutant enzyme T206G and wild-type enzyme | Alcaligenes faecalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | catalytically active enzyme contains one tightly bound Ca2+ | Alcaligenes faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme is maturated in vivo from an inactive precursor into the catalytically active enzyme, via a complex post-translational autocatalytic processing with a multi-step excision of a small internal pro-peptide | Alcaligenes faecalis |
Purification (Comment) | Organism |
---|---|
wild-type enzyme and pro-peptide mutants T206G, T206P, T206G/G213G and T206G/S213G/T219G | Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylpenicillin + H2O | - |
Alcaligenes faecalis | phenylacetic acid + 6-aminopenicillanate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80 | - |
benzylpenicillin | pH 7.5, 25°C, wild-type enzyme | Alcaligenes faecalis | |
120 | - |
benzylpenicillin | pH 7.5, 25°C, mutant enzyme T206G | Alcaligenes faecalis | |
140 | - |
benzylpenicillin | pH 7.5, 25°C, mutant enzyme T206G//S213G | Alcaligenes faecalis | |
190 | - |
benzylpenicillin | pH 7.5, 25°C, mutant enzyme T206G/S213G/T219G | Alcaligenes faecalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Alcaligenes faecalis | wild-type enzyme and pro-peptide mutant T206P, isoelectric focusing | - |
5.3 |
Alcaligenes faecalis | pro-peptide mutant enzymes T206G and T206G/S213G/T219G, isoelectric focusing | - |
5.5 |
Alcaligenes faecalis | pro-peptide mutant enzyme T206G/S213G, isoelectric focusing | 5.9 | 5.6 |