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Literature summary for 3.5.1.11 extracted from

  • Ignatova, Z.; Stoeva, S.; Galunsky, B.; Hörnle, C.; Nurk, A.; Piotraschke, E.; Voelter, W.; Kasche, V.
    Proteolytic processing of penicillin amidase from Alcaligenes faecalis cloned in Escherichia coli yields several active forms (1998), Biotechnol. Lett., 20, 977-982.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Alcaligenes faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
benzylpenicillin pH 7.5, 25°C, enzyme form PA5..5 and PA5.3 Alcaligenes faecalis
0.01
-
benzylpenicillin pH 7.5, 25°C Escherichia coli
0.012
-
6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C, enzyme form PA5.3 Alcaligenes faecalis
0.017
-
6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C, enzyme form PA5.5 Alcaligenes faecalis
0.02
-
6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22540
-
? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI Alcaligenes faecalis
22920
-
? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI Alcaligenes faecalis
62564
-
? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI Alcaligenes faecalis
62568
-
? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme expressed in Escherichia coli Alcaligenes faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
3.8 DELTAA380/min per absorbance of the enzyme solution at 280 nM for enzyme form PA5.3 and 3.2 DELTAA380/min per absorbance of the enzyme solution at 280 nM for enzyme form PA5.5 Alcaligenes faecalis

Storage Stability

Storage Stability Organism
4 °C, half-life is 6 h Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-nitro-3-phenylacetamidobenzoic acid + H2O
-
Alcaligenes faecalis ?
-
?
6-nitro-3-phenylacetamidobenzoic acid + H2O
-
Escherichia coli ?
-
?
benzylpenicillin + H2O
-
Alcaligenes faecalis phenylacetic acid + 6-aminopenicillanate
-
?
benzylpenicillin + H2O
-
Escherichia coli phenylacetic acid + 6-aminopenicillanate
-
?

Subunits

Subunits Comment Organism
? ? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI Alcaligenes faecalis
? ? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI Alcaligenes faecalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
half-life: 6 h Alcaligenes faecalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C Escherichia coli
50
-
benzylpenicillin pH 7.5, 25°C Escherichia coli
75
-
benzylpenicillin pH 7.5, 25°C, enzyme form PA5..5 Alcaligenes faecalis
77
-
6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C, enzyme form PA5..5 Alcaligenes faecalis
80
-
benzylpenicillin pH 7.5, 25°C, enzyme form PA5..3 Alcaligenes faecalis
82
-
6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C, enzyme form PA5..3 Alcaligenes faecalis

pI Value

Organism Comment pI Value Maximum pI Value
Alcaligenes faecalis enzyme form PA5.3, isoelectric focusing
-
5.3
Alcaligenes faecalis enzyme form PA5.5, isoelectric focusing
-
5.5
Escherichia coli isoelectric focusing
-
7