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Literature summary for 3.5.1.11 extracted from
- A simplified determination of penicillin amidase from E. coli (1971), Hoppe-Seyler's Z. Physiol. Chem., 352, 1723-1724.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | synthesis of 6-aminopenicillanate | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | Escherichia coli | - |
6-aminopenicillanic acid + phenylacetic acid | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
6-aminopenicillanic acid, pH 8, 27°C | Escherichia coli |
| 6.3 | - |
6-aminopenicillanic acid, pH 7 | Escherichia coli |
| 6.6 | - |
6-aminopenicillanic acid, pH 7.5 | Escherichia coli |
| 7.1 | - |
6-aminopenicillanic acid, pH 8-8.5 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanate + phenylacetic acid | - |
r | |
| penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| phenylacetyl-L-asparagine + H2O | - |
Escherichia coli | L-asparagine + phenylacetic acid | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Escherichia coli |
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