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Literature summary for 3.5.1.108 extracted from

  • Whittington, D.A.; Rusche, K.M.; Shin, H.; Fierke, C.A.; Christianson, D.W.
    Crystal structure of LpxC, a zinc-dependent deacetylase essential for endotoxin biosynthesis (2003), Proc. Natl. Acad. Sci. USA, 100, 8146-8150.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.108

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization at 21°C, a sitting drop containing 0.005 ml of protein solution (2.2 mg/ml LpxC, 25 mM Hepes (pH 7.0), 50 mM NaCl, 10 mM magnesium acetate, and 0.5 mM ZnSO4) is equilibrated against a 0.5 ml reservoir of 0.8 M NaCl/0.1 M Hepes (pH 7.0). Crystals of dimensions 0.3 * 0.1 * 0.05 mm3 appear in 5-7 days, larger crystals of dimensions 0.6 * 0.2 * 0.2 mm3 are obtained by macroseeding Aquifex aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-binding motif. The catalytic zinc ion resides at the base of an active-site cleft and adjacent to a hydrophobic tunnel occupied by a fatty acid Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O67648
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Purification (Commentary)

Purification (Comment) Organism
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 Aquifex aeolicus

Synonyms

Synonyms Comment Organism
LpxC
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 Aquifex aeolicus
UDP-(3-O-(R-3-hydroxymyristoyl))-N-acetylglucosamine deacetylase
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 Aquifex aeolicus