Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
D125A | mutation leads to a complete loss of the deformylase activity | Pseudomonas putida |
E221A | 70% of wild-type deformylase acticity | Pseudomonas putida |
H245A | mutation leads to a complete loss of the deformylase activity | Pseudomonas putida |
S101A | mutation leads to a complete loss of the deformylase activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE | Pseudomonas putida |
29100 | - |
x * 29100, calculated from sequence | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formylmaleamic acid + H2O | Pseudomonas putida | aerobic catabolism of nicotinic acid | maleamate + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida KT 2240 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formylmaleamic acid + H2O | aerobic catabolism of nicotinic acid | Pseudomonas putida | maleamate + formate | - |
? | |
N-formylmaleamic acid + H2O | S101, D125, and H245 are essential for the enzyme activity, constituting the catalytic triad of the NicD deformylase | Pseudomonas putida | maleamate + formate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Pseudomonas putida |
? | x * 29100, calculated from sequence | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
NicD | - |
Pseudomonas putida |