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Literature summary for 3.5.1.105 extracted from

  • Ohishi, K.; Yamagishi, M.; Ohta, T.; Motosugi, M.; Izumida, H.; Sano, H.; Adachi, K.; Miwa, T.
    Purification and properties of two deacetylases produced by Vibrio alginolyticus H-8 (1997), Biosci. Biotechnol. Biochem., 61, 1113-1117.
No PubMed abstract available
Search for more literature for 3.5.1.105

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM AgNO3, complete inhibition Vibrio alginolyticus
Al3+ 1 mM AlCl3, 30% inhibition Vibrio alginolyticus
Cu2+ 1 mM CuCl2, 53% inhibition Vibrio alginolyticus
Fe2+ 1 mM FeSO4, 26% inhibition Vibrio alginolyticus
Hg2+ 1 mM HgCl2, complete inhibition Vibrio alginolyticus
Sn2+ 1 mM SnCl2, 44% inhibition Vibrio alginolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, SDS-PAGE Vibrio alginolyticus

Organism

Organism UniProt Comment Textmining
Vibrio alginolyticus Q99PX1
-
-
Vibrio alginolyticus H-8 Q99PX1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio alginolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32.2
-
-
 Vibrio alginolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNAc-beta-(1-4)-GlcNAc + H2O i.e. 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-D-glucopyranose. No activity with chitotriose, chitotetraose, chitopentaose and chitohexaose. The 2-acetamido group is completely hydrolyzed within 3 h, no hydrolysis of 2'-acetamide group occurs Vibrio alginolyticus GlcNAc-beta-(1-4)-GlcN + acetate i.e. 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine ?
GlcNAc-beta-(1-4)-GlcNAc + H2O i.e. 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-D-glucopyranose. No activity with chitotriose, chitotetraose, chitopentaose and chitohexaose. The 2-acetamido group is completely hydrolyzed within 3 h, no hydrolysis of 2'-acetamide group occurs Vibrio alginolyticus H-8 GlcNAc-beta-(1-4)-GlcN + acetate i.e. 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Vibrio alginolyticus

Synonyms

Synonyms Comment Organism
deacetylase DA1
-
 Vibrio alginolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Vibrio alginolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 about 50% of maximal activity at 30°C and 50°C Vibrio alginolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 7.5, 30 min, about 10% loss of activity Vibrio alginolyticus
50
-
 pH 7.5, 30 min, about 40% loss of activity Vibrio alginolyticus
70
-
 pH 7.5, 30 min, about 80% loss of activity Vibrio alginolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Vibrio alginolyticus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 11 pH 6.5: about 45% of maximal activity, pH 11: about 55% of maximal activity Vibrio alginolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 37°C, 20 min, about 75% loss of activity Vibrio alginolyticus
5
-
 37°C, 20 min, about 40% loss of activity Vibrio alginolyticus
7 11 37°C, 20 min, stable Vibrio alginolyticus

pI Value

Organism Comment pI Value Maximum pI Value
Vibrio alginolyticus isoelectric focusing
-
 3.3