Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the active site metal ion is coordinated to two histidine residues, His13 and His147, and an aspartate, Asp16 | Mycobacterium tuberculosis | |
Zn2+ | the active site metal ion is coordinated to two histidine residues, His13 and His147, and an aspartate, Asp16 | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WJN3 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H2O = 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate | proposed catalytic mechanism of MshB, overview. MshB uses a general acid-base mechanism in which Asp15 and His144 act as the general base and acid catalysts respectively, while Tyr142 plays a dynamic role that includes stabilization of the oxyanion intermediate | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | structural analogues of N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-D-glucopyranoside in which the inosityl moiety is replaced by a chromophore are synthesized and evaluated as alternate substrates of MshB, assay method optimization for high-throughput measurements, molecular docking analysis of the MshB active site binding pocke, overview | Mycobacterium tuberculosis | ? | - |
? | |
N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-D-glucopyranoside + H2O | molecular docking analysis of the MshB active site binding pocket, overview | Mycobacterium tuberculosis | 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate | - |
? | |
N-acetyl-D-glucosamine + H2O | commercially available alternate minimal substrate, MshB is 100fold less active towards this compound than toward the natural substrate | Mycobacterium tuberculosis | acetate + D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlcNAc-Ins deacetylase | - |
Mycobacterium tuberculosis |
MshB | - |
Mycobacterium tuberculosis |
N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-D-glucopyranoside deacetylase | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of mycothiol (MSH), a protective low molecular weight thiol present only in Mycobacterium tuberculosis and other actinomycetes | Mycobacterium tuberculosis |