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Literature summary for 3.5.1.1 extracted from
- Expression and functional characterization of Pseudomonas aeruginosa recombinant L.asparaginase (2018), Protein J., 37, 461-471 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | purified L-asparaginase does not show hemolysis effect on blood erythrocytes. Recombinant L-asparaginase retains 50% of its initial activity after 90 and 60 min incubation in serum and trypsin separately | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular modeling of structure | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 5 mM, 82% of initial activity | Pseudomonas aeruginosa |  |
| Zn2+ | 5 mM, 72% of initial activity | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, 122% of initial activity | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72400 | - |
PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5925 | - |
pH not specified in the publication, temperature not specified in the publication | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acrylamide + H2O | - |
Pseudomonas aeruginosa | acrylic acid + NH3 | - |
? | |
| L-asparagine + H2O | - |
Pseudomonas aeruginosa | L-aspartate + NH3 | - |
? | |
| L-glutamine + H2O | reaction of EC 3.5.1.2 | Pseudomonas aeruginosa | L-glutamate + NH3 | - |
? | |
| urea + H2O | - |
Pseudomonas aeruginosa | ? + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36300, SDS-PAGE, 2 * 36276, calculated from sequence | Pseudomonas aeruginosa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas aeruginosa | calculated from sequence | - |
4.7 |
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